Song Jingze, Li Na, Dong Xinze, Xie Jianping, Lai Hongqiao, Zhu Hengzhi, He Kongwang, Wen Libin, Suolang Sizhu, Xiao Qi
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China.
Curr Issues Mol Biol. 2025 Jul 18;47(7):566. doi: 10.3390/cimb47070566.
This study investigated the isoforms of porcine-origin Ral guanine nucleotide dissociation stimulator (RalGDS) in LLC-PK1 cells using reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Through segmented amplification, sequence assembly, and comparative genomics analysis, seven RalGDS isoforms were identified, characterized by insertions, deletions, and frameshift mutations. These genetic variations may significantly alter RalGDS's protein structure and function, potentially impacting its role in Ral GTPase-mediated signaling pathways. This work provides foundational insights into the genetic diversity of porcine RalGDS and its implications for porcine physiology and economically significant traits.
本研究利用逆转录-聚合酶链反应（RT-PCR）和测序技术，对LLC-PK1细胞中猪源Ral鸟嘌呤核苷酸解离刺激因子（RalGDS）的亚型进行了研究。通过分段扩增、序列组装和比较基因组学分析，鉴定出了7种RalGDS亚型，其特征为插入、缺失和移码突变。这些基因变异可能会显著改变RalGDS的蛋白质结构和功能，进而可能影响其在Ral GTP酶介导的信号通路中的作用。这项工作为猪RalGDS的遗传多样性及其对猪生理学和经济重要性状的影响提供了基础见解。
