Zhang Jingzhe, Wang Xinkun, Fu Xinbiao, Li Ye
Department of Orthopedics, Jilin University Sino Japanese Friendship Hospital, Changchun, 130000, China.
Jilin University Sino Japanese Friendship Hospital, No. 126, Xiantai Street, Changchun, 130000, China.
J Biol Eng. 2025 Jul 29;19(1):72. doi: 10.1186/s13036-025-00529-5.
Bone regeneration requires innovative solutions to enhance osteogenic differentiation and support effective tissue repair. This study presents a novel approach to bone tissue engineering by developing peptide-functionalized nanofibrous scaffolds (NFS). The fabrication of a blended hyaluronic acid (HA) and polydopamine (PD) scaffold functionalized with bone marrow mesenchymal stem cells (BMSCs)-affinity peptides (AP) designed to control magnesium ion (Mg) release, which supports BMSCs' osteogenic differentiation and bone regeneration. Characterization studies, including fourier-transform infrared spectroscopy (FTIR) and morphological analysis, confirmed the hydrophilic properties of HA/PDNFS@BMSCs-AP scaffolds, which enhance cell adhesion and proliferation. In vitro and In vivo assessments revealed that the scaffolds significantly promote osteogenesis through AP-induced pathways such as extracellular signal-regulated kinase pathway (ERK) and Phosphatidylinositol 3-kinase (Akt),. Animal model experiments demonstrated accelerated bone repair, supporting the potential of HA/PDNFS@BMSCs-AP scaffolds for targeted bone defect healing. These findings highlight the promise of functionalized nanofibrous scaffolds in bone tissue engineering and their potential application in regenerative medicine and translational research.
骨再生需要创新的解决方案来增强成骨分化并支持有效的组织修复。本研究通过开发肽功能化纳米纤维支架（NFS），提出了一种骨组织工程的新方法。制备了一种混合透明质酸（HA）和聚多巴胺（PD）的支架，该支架用骨髓间充质干细胞（BMSCs）亲和肽（AP）功能化，旨在控制镁离子（Mg）释放，以支持BMSCs的成骨分化和骨再生。包括傅里叶变换红外光谱（FTIR）和形态分析在内的表征研究证实了HA/PDNFS@BMSCs-AP支架的亲水性，这增强了细胞粘附和增殖。体外和体内评估表明，该支架通过AP诱导的细胞外信号调节激酶途径（ERK）和磷脂酰肌醇3激酶（Akt）等途径显著促进成骨。动物模型实验证明了骨修复加速，支持了HA/PDNFS@BMSCs-AP支架在靶向骨缺损愈合方面的潜力。这些发现突出了功能化纳米纤维支架在骨组织工程中的前景及其在再生医学和转化研究中的潜在应用。
