Borza Tudor, Lumactud Rhea Amor, Shim So Yeon, Al-Mughrabi Khalil, Prithiviraj Balakrishnan
Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, 50 Pictou Road, Cox Institute, Truro, NS B2N 5E3, Canada.
School of Climate Change and Adaptation, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.
Microorganisms. 2025 Jun 26;13(7):1482. doi: 10.3390/microorganisms13071482.
Potato early dying disease complex (PED) leads to premature senescence and rapid decline in potato plants. Unlike potato wilt caused solely by species, PED symptoms are more severe due to the synergistic effects of multiple pathogens, including root-lesion nematodes, fungi such as and , and soft-rot bacteria. To investigate the microbiome responsible for PED, soil and stem samples from healthy-looking and symptomatic plants were analyzed using amplicon-targeted next-generation sequencing (Illumina MiSeq and PacBio technologies). Samples were collected from four locations in New Brunswick, Canada from fields previously rotated with barley or oat. Comparative analysis of the bacterial, fungal, and eukaryotic diversity in soil samples showed minimal differences, with only bacterial alpha diversity influenced by the plant health status. was abundant in all soil samples, and its abundance was significantly higher in the stems of diseased plants. Additional fungal species implicated in PED, including , , sp., and , were also identified in the stems. This study highlights the complex, plant-associated microbial interactions underlying PED and provides a foundation for microbiome-informed disease management strategies.
马铃薯早死病复合体（PED）会导致马铃薯植株过早衰老并迅速衰退。与仅由单一物种引起的马铃薯枯萎病不同，由于多种病原体的协同作用，PED的症状更为严重，这些病原体包括根腐线虫、真菌（如和）以及软腐细菌。为了研究导致PED的微生物群落，我们使用靶向扩增子的新一代测序技术（Illumina MiSeq和PacBio技术）对外观健康和有症状植株的土壤和茎样本进行了分析。样本取自加拿大新不伦瑞克省的四个地点，这些田地之前轮作过大麦或燕麦。对土壤样本中细菌、真菌和真核生物多样性的比较分析显示差异极小，只有细菌的α多样性受植株健康状况影响。在所有土壤样本中都很丰富，并且在患病植株的茎中其丰度显著更高。在茎中还鉴定出了其他与PED相关的真菌物种，包括、、某物种以及。这项研究突出了PED背后复杂的、与植物相关的微生物相互作用，并为基于微生物群落的病害管理策略提供了基础。
