Harnessing Waste Bread: From Potential Use in Microbial Growth and Enzyme Production to Techno-Economic Assessment.

This study highlights waste bread (WB) as a novel, cost-effective, and nutrient-rich substrate for microbial growth, offering a sustainable alternative to conventional media. As a renewable resource, WB promotes the circular economy by reducing food waste and encouraging biotechnological innovation. The incorporation of WB into microbial culture media enhanced the growth of various reference strains (, , , and ), with at least a two-fold increase compared to conventional Luria-Bertani (LB) medium. Moreover, combining 2% WB with diluted LB (1/10) reduced medium costs by up to 90%. Furthermore, it was confirmed that 1% WB can effectively replace starch during the screening of amylolytic strains. Applying a fractional factorial design, the production of amylase by sp. BSS (Amy-BSS) was enhanced 15-fold. An analysis of the Pareto diagram revealed that WB was the most significant factor. Additionally, Amy-BSS was applied to hydrolyze polysaccharides in WB, enabling the generation of high-value-added products in food processing. This hydrolysis process yielded 4.6 g/L of fermentable sugars from 1% WB. Evaluating the economic feasibility of WB valorization into value-added products elucidates potential pathways for cost reduction and enhanced environmental sustainability, thereby positioning WB as a viable tool for sustainable development.