Physiological tremor analysis is a practical tool for assessing the neuromuscular impacts of sport-specific training. The purpose of this study was to examine and compare the physiological characteristics of lower limb resting postural tremor in athletes from Poland's national speed skating team, following both sprint and endurance workouts. The study included 19 male, well-trained, elite athletes (with a mean age of 18 ± 3.1 years, body mass of 71.4 ± 10.1 kg, height of 178.5 ± 9.0 cm, and training experience of 12.6 ± 2.8 years) and a control group of 19 physically active but non-athlete men (with a mean age of 19 ± 2.3 years, body mass of 78.9 ± 12.1 kg, and height of 181.5 ± 11.0 cm). This group was assessed under resting conditions to provide baseline reference values for physiological tremor and to evaluate whether the neuromuscular tremor response is specific to trained athletes. Tremor amplitude and frequency were measured using an accelerometer, with data log-transformed to normalize the power spectrum distribution. Key findings indicate a significant effect of training condition on tremor amplitude in the low-frequency range (L(2_5); F = 38.42; < 0.0001; η = 0.68) and high-frequency range (L(9_14); F = 19.19; < 0.0001; η = 0.51). Post hoc analysis showed that tremor amplitude increased significantly after both sprint ( < 0.001) and endurance training ( < 0.001) compared to rest. No significant differences were observed between sprint and endurance training conditions for L(2_5) ( = 0.1014), but sprint training resulted in a greater increase in tremor in the high-frequency range (L(9_14); < 0.0001). Tremor frequency (F(2_5) and F(9_14)) also increased significantly post-training. Notably, no differences were observed between limbs, indicating symmetrical neuromuscular adaptation. These findings highlight the utility of tremor analysis in monitoring neuromuscular fatigue and performance in speed skaters. Future research should explore the application of this method in broader athletic populations and evaluate its potential integration into training programs.