Vasilyeva A D, Yurina L V, Evtushenko E G, Gavrilina E S, Krylov V B, Nifantiev N E, Kurochkin I N
N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia.
Faculty of Chemistry, Lomonosov Moscow State University, 1/3 Leninskie Gory, 119991 Moscow, Russia.
Sensors (Basel). 2025 Jul 13;25(14):4376. doi: 10.3390/s25144376.
Galactomannan (GM) is a polysaccharide secreted by opportunistic pathogenic fungi of the Aspergillus genus. It is prescribed as a diagnostic biomarker of invasive aspergillosis in immunocompromised patients by the guidelines for diagnosis and management of Aspergillus diseases. It has been shown previously that the measurement of soluble horseradish peroxidase (HRP) using surface-enhanced Raman scattering (SERS) of 2,3-diaminophenazine enzymatic reaction product on silver nanoparticles is largely superior in detection limit compared to colorimetric readout. In this study, a highly sensitive SERS-based HRP measurement protocol was applied to enzyme-linked immunosorbent assay (ELISA) for GM quantification in biological fluids. The detection limit for GM was 4.3 pg per sample, which is one and a half orders of magnitude lower compared to colorimetric detection with o-phenylenediamine as a substrate and five times more sensitive than ELISA using 3,3',5,5'-tetramethylbenzidine.
半乳甘露聚糖（GM）是曲霉属机会性致病真菌分泌的一种多糖。根据曲霉病诊断和管理指南，它被用作免疫功能低下患者侵袭性曲霉病的诊断生物标志物。先前已经表明，使用银纳米颗粒上2,3-二氨基吩嗪酶促反应产物的表面增强拉曼散射（SERS）来测量可溶性辣根过氧化物酶（HRP），与比色读数相比，在检测限方面有很大优势。在本研究中，一种基于SERS的高灵敏度HRP测量方案被应用于酶联免疫吸附测定（ELISA），用于生物体液中GM的定量。GM的检测限为每个样品4.3皮克，与以邻苯二胺为底物的比色检测相比低一个半数量级，比使用3,3',5,5'-四甲基联苯胺的ELISA灵敏度高五倍。
