Angsusing Jaenjira, Samee Weerasak, Mangmool Supachoke, Dortae Usma, Keawthip Pranot, Mahasirimongkol Surakameth, Kreechai Somsak, Wanaratna Kulthanit, Chittasupho Chuda, Toolmal Nopparut
Department of Thai Traditional and Alternative Medicine, Ministry of Public Health, Nonthaburi 11000, Thailand.
Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand.
Pharmaceutics. 2025 Jul 13;17(7):907. doi: 10.3390/pharmaceutics17070907.
: This study aimed to investigate the antioxidant and anti-inflammatory properties of a Hand and Foot Soaking Formulary composed of ten medicinal plants, with curcumin as a major bioactive marker, to provide scientific validation for its traditional use. The formulation was evaluated for total phenolic and flavonoid contents, with curcumin quantified using HPLC. Antioxidant activity was assessed using DPPH, ABTS, and FRAP assays. Cytotoxicity was evaluated in RAW264.7 cells using the MTT assay. Anti-inflammatory activity was determined by measuring nitric oxide (NO), PGE, TNF-α, IL-1β, and IL-6 levels in LPS-stimulated RAW264.7 macrophages using ELISA. : The Hand and Foot Soaking Formulary exhibited promising antioxidant and anti-inflammatory properties, consistent with its traditional use. Phytochemical analysis confirmed the presence of bioactive compounds, with measurable levels of total phenolics, flavonoids, and significant curcumin content. Antioxidant activity was demonstrated through free radical scavenging and ferric-reducing assays, while cytotoxicity testing in RAW264.7 macrophages indicated low toxicity (IC = 48.61 ± 3.80 µg/mL). The formulary significantly reduced LPS-induced nitric oxide, PGE, TNF-α, IL-1β, and IL-6 production. These effects were comparable to turmeric extract and curcumin, though curcumin displayed higher potency. : The Hand and Foot Soaking Formulary demonstrates antioxidant and anti-inflammatory properties in vitro, supporting its traditional use. Its polyherbal composition may offer synergistic effects and holds promise as a safe, natural topical remedy.
本研究旨在调查一种由十种药用植物组成的手足浸泡配方的抗氧化和抗炎特性，以姜黄素作为主要生物活性标志物，为其传统用途提供科学验证。对该配方进行了总酚和黄酮含量评估，使用高效液相色谱法对姜黄素进行定量。使用DPPH、ABTS和FRAP测定法评估抗氧化活性。使用MTT测定法在RAW264.7细胞中评估细胞毒性。通过酶联免疫吸附测定法测量脂多糖刺激的RAW264.7巨噬细胞中的一氧化氮（NO）、前列腺素E（PGE）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平来确定抗炎活性。
该手足浸泡配方表现出有前景的抗氧化和抗炎特性，与其传统用途一致。植物化学分析证实了生物活性化合物的存在，总酚、黄酮含量可测，且姜黄素含量显著。通过自由基清除和铁还原测定法证明了抗氧化活性，而在RAW264.7巨噬细胞中的细胞毒性测试表明毒性较低（半数抑制浓度=48.61±3.80μg/mL）。该配方显著降低了脂多糖诱导的一氧化氮、前列腺素E、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6的产生。这些效果与姜黄提取物和姜黄素相当，不过姜黄素表现出更高的效力。
该手足浸泡配方在体外表现出抗氧化和抗炎特性，支持其传统用途。其多草药成分可能具有协同作用，有望成为一种安全、天然的局部治疗药物。
