Pokorski Patryk, Strojny-Cieślak Barbara, Domian Ewa, Załęcki Michał, Grygier Anna, Pruchniewski Michał, Zakrzewska Anna, Aktaş Havva, Aljewicz Marek, Kmiecik Dominik, Custodio-Mendoza Jorge A, Boruszewski Piotr, Kurek Marcin A
Department of Technique and Food Product Development, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Carbohydr Polym. 2025 Oct 15;366:123892. doi: 10.1016/j.carbpol.2025.123892. Epub 2025 Jun 10.
This study investigates the application of edible insect protein-chitosan (IP/CS) complex coacervates, derived from T. molitor, A. domesticus, and L. migratoria proteins combined with low-, medium-, or high-molecular-weight chitosans, as stabilizers in Pickering emulsions aimed at enhancing emulsion stability and improving the retention of bioactive compounds. The underlying hypothesis posits that IP/CS can function as effective stabilizing systems in Pickering emulsions, providing high overall stability, low susceptibility to destabilization, and efficient encapsulation of lipophilic bioactives. Experimental results showed that IP/CS-based Pickering emulsions achieved high β-sitosterol retention, ranging from 56 % to 91 %. Emulsion stability was primarily attributed to electrostatic interactions and hydrogen bonding within the coacervates. In addition, an effective stabilization mechanism was identified, involving the physical integration of oil droplets into the IP/CS matrix, which contributed to reduced flocculation. Fourier-transform infrared (FTIR) spectroscopy and rheological analysis confirmed the presence of hydrogen bonding and indicated a viscous character of the emulsions, while ζ-potential measurements revealed surface charge properties that further supported emulsion stabilization. These findings demonstrate the role of intermolecular interactions in maintaining the integrity of oil-in-water (O/W) Pickering emulsions stabilized by IP/CS complexes.
本研究调查了源自黄粉虫、家蝇和东亚飞蝗蛋白质与低、中、高分子量壳聚糖结合而成的可食用昆虫蛋白-壳聚糖（IP/CS）复合凝聚层作为皮克林乳液稳定剂的应用，旨在提高乳液稳定性并改善生物活性化合物的保留率。基本假设认为，IP/CS可作为皮克林乳液中的有效稳定体系，提供高整体稳定性、低失稳敏感性以及对亲脂性生物活性物质的有效包封。实验结果表明，基于IP/CS的皮克林乳液实现了56%至91%的高β-谷甾醇保留率。乳液稳定性主要归因于凝聚层内的静电相互作用和氢键。此外，还确定了一种有效的稳定机制，涉及油滴物理整合到IP/CS基质中，这有助于减少絮凝。傅里叶变换红外（FTIR）光谱和流变学分析证实了氢键的存在，并表明乳液具有粘性特征，而ζ电位测量揭示的表面电荷性质进一步支持了乳液的稳定。这些发现证明了分子间相互作用在维持由IP/CS复合物稳定的水包油（O/W）皮克林乳液完整性中的作用。
