一种用于评估抗柯萨奇病毒A4抗病毒试剂的致死性幼年小鼠模型。
A lethal juvenile mouse model for the evaluation of antiviral reagents against coxsackievirus A4.
作者信息
Su Qin, Chen Xiao, Zheng Zhihui, Wei Hailin, Zhou Jiaxue, Zhang Libing, Muhammad Ali, Wang Yin, Chen Xiang, Zhang Pinghu
机构信息
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225009, China.
Emergency Intensive Care Unit (EICU) and Department of Emergency Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu 225002, China.
出版信息
iScience. 2025 Jun 30;28(8):113022. doi: 10.1016/j.isci.2025.113022. eCollection 2025 Aug 15.
Coxsackievirus A4 (CVA4) as a pathogen causing severe herpangina has become global outbreak epidemics during the past 5 years. However, there is currently no effective vaccines and drugs available. Here, one mouse-adapted CVA4 virus (YZ08) was cultivated by repeatedly cross-passaging between human RD cells and 11-day-old juvenile mice. Our results revealed the YZ08 virus was highly lethal to 13-day-old juvenile mice. Virus titer assay indicated that the YZ08 virus effectively replicated in juvenile mice. This model was successfully used to evaluate the therapeutic efficacy of herb medicine, anti-CVA4 rabbit sera, and antiviral drugs. Our results indicated that all tested reagents effectively provided protection for CVA4 virus infection by prolonging survival time, promoting survival, and preventing weight loss. Collectively, we successfully established a juvenile mouse model of CVA4 virus, which was a powerful tool for investigating the pathogenesis of CVA4 or evaluating the efficacy of antiviral reagents or vaccines.
柯萨奇病毒A4（CVA4）作为引起严重疱疹性咽峡炎的病原体，在过去5年中已在全球爆发流行。然而，目前尚无有效的疫苗和药物。在此，通过在人RD细胞和11日龄幼鼠之间反复传代培养出一种适应小鼠的CVA4病毒（YZ08）。我们的结果显示，YZ08病毒对13日龄幼鼠具有高度致死性。病毒滴度测定表明，YZ08病毒能在幼鼠体内有效复制。该模型成功用于评估草药、抗CVA4兔血清和抗病毒药物的治疗效果。我们的结果表明，所有测试试剂均通过延长存活时间、促进存活和防止体重减轻，有效地为CVA4病毒感染提供了保护。总体而言，我们成功建立了CVA4病毒幼鼠模型，这是研究CVA4发病机制或评估抗病毒试剂或疫苗疗效的有力工具。
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