Roughley P J, Mort J S
Anal Biochem. 1985 Aug 15;149(1):136-41. doi: 10.1016/0003-2697(85)90486-5.
Cartilage proteoglycan subunits are resolved from their various-size proteolytic degradation products by a gel filtration high-performance liquid chromatography system using a Bio-Gel TSK-60 column in tandem with a Bio-Gel TSK-50 column. Molecules ranging in size from the intact proteoglycan to single chondroitin sulfate chains are eluted in the included volume. Each analysis takes less than 30 min to complete, and with purified samples as little as 20 micrograms of proteoglycan is required. The method can be applied to the measurement of proteoglycan in mixtures, such as tissue culture media, by monitoring effluent fractions using the dimethylmethylene blue dye-binding assay.
通过凝胶过滤高效液相色谱系统，使用串联的Bio-Gel TSK-60柱和Bio-Gel TSK-50柱，可从各种大小的蛋白水解降解产物中分离出软骨蛋白聚糖亚基。大小从完整蛋白聚糖到单个硫酸软骨素链的分子在包容体积中被洗脱。每次分析完成时间不到30分钟，对于纯化样品，所需蛋白聚糖低至20微克。该方法可通过使用二甲基亚甲基蓝染料结合测定法监测流出级分，应用于测量混合物（如组织培养基）中的蛋白聚糖。
