Lequeu B, Guilland J C, Klepping J
Anal Biochem. 1985 Sep;149(2):296-300. doi: 10.1016/0003-2697(85)90573-1.
An assay method for the determination of pyridoxal 5'-phosphate (PLP) in plasma is presented. The procedure is rapid and requires only a small volume. The method includes PLP-dependent enzymatic decarboxylation of L-tyrosine to tyramine (enzyme used: L-tyrosine apodecarboxylase (EC 4.1.1.25) from Streptococcus faecalis) and measurement of tyramine by high-performance liquid chromatography with electrochemical detection. This technique confers specificity, rapidity, and low-cost measurement. Human plasma PLP from 30 normal healthy adults had a mean value of 65.10 +/- 4.70 nM. Sensitivity of the reaction was 1.3 nM and the coefficient of variation of the method (30 repeated assays of sample with a value of 60 nM) was 1.9%.
介绍了一种测定血浆中磷酸吡哆醛（PLP）的分析方法。该方法快速，且仅需少量样本。该方法包括依赖PLP的L-酪氨酸酶促脱羧生成酪胺（所用酶：粪肠球菌的L-酪氨酸脱羧酶（EC 4.1.1.25）），并通过高效液相色谱-电化学检测法测定酪胺。该技术具有特异性、快速性和低成本测量的特点。30名正常健康成年人的人血浆PLP平均值为65.10 +/- 4.70 nM。该反应的灵敏度为1.3 nM，该方法的变异系数（对值为60 nM的样本进行30次重复测定）为1.9%。
