Liu Bingxiao, Wang Zhengxuan, Liang Mingcai, Yang Lin
School of Chemistry and Chemical Engineering Harbin Institute of Technology Harbin China.
Food Sci Nutr. 2025 Jul 28;13(8):e70687. doi: 10.1002/fsn3.70687. eCollection 2025 Aug.
The present study investigated the effect of rice protein (RP) on plasma cholesterol clearance by administering diets containing either casein or RP to growing and adult male Wistar rats over a two-week period. RP intake markedly upregulated the gene and protein expression of key regulators involved in cholesterol transport and metabolism, including ATP-binding cassette transporter G1 (ABCG1), scavenger receptor class B type I (SR-BI), ABCG5, bile salt export pump (BSEP), coactivator-associated arginine methyltransferase 1 (CARM1), and farnesoid X receptor (FXR), in both age groups. These findings indicate that RP effectively promotes cholesterol and bile acid excretion. Consequently, RP significantly reduced cholesterol concentrations in both plasma and liver ( < 0.05), while fecal excretion of cholesterol and bile acids was significantly increased ( < 0.05). Overall, the results reveal a clear association between the cholesterol-lowering effects of RP and its regulatory role in cholesterol elimination, irrespective of age. Notably, the ability of RP to stimulate the expression of ABCG1, low-density lipoprotein receptor (LDLR), SR-BI, ABCG5, BSEP, CARM1, and FXR remains unaffected by aging, suggesting a consistent hypocholesterolemic effect across different life stages.
本研究通过在两周时间内给生长中的和成年雄性Wistar大鼠喂食含有酪蛋白或大米蛋白（RP）的日粮，研究了大米蛋白（RP）对血浆胆固醇清除的影响。在两个年龄组中，摄入RP均显著上调了参与胆固醇转运和代谢的关键调节因子的基因和蛋白表达，包括ATP结合盒转运体G1（ABCG1）、B类I型清道夫受体（SR-BI）、ABCG5、胆盐输出泵（BSEP）、共激活因子相关精氨酸甲基转移酶1（CARM1）和法尼醇X受体（FXR）。这些发现表明，RP能有效促进胆固醇和胆汁酸的排泄。因此，RP显著降低了血浆和肝脏中的胆固醇浓度（<0.05），而胆固醇和胆汁酸的粪便排泄量显著增加（<0.05）。总体而言，结果揭示了RP的降胆固醇作用与其在胆固醇消除中的调节作用之间存在明显关联，且与年龄无关。值得注意的是，RP刺激ABCG1、低密度脂蛋白受体（LDLR）、SR-BI、ABCG5,BSEP、CARM1和FXR表达的能力不受衰老影响，这表明在不同生命阶段RP都具有一致的降胆固醇作用。
