Hubbard C J, Greenwald G S
Anat Rec. 1985 Aug;212(4):353-7. doi: 10.1002/ar.1092120405.
Hypophysectomized female hamsters primed with 30 i.u. pregnant mare serum gonadotropin (PMSG) were injected with anti-PMSG serum 72 h later to induce atresia in Graafian follicles. The earliest histological evidence of atresia was seen in the cumulus oophorus at 4 h with a significant increase in pyknotic nuclei. The mural granulosa cells, however, did not show pyknoses until 12 h following anti-PMSG serum. The highest number of pyknotic nuclei in the cumulus oophorus was seen at 24 h, when pseudomaturation of the oocyte occurred. Breakdown of the mural granulosa cells was greatest by 48 h. Morphometric measurements of the follicles showed a significant decline in the thickness and area by the granulosa layer at 48 h following injection of anti-PMSG serum. The thecal compartment showed a decline in number of mitoses at 4 h and 12 h and in area at 48 h. However, no pyknotic thecal cells were observed during the 72 h following injection of anti-PMSG serum. The results support previous work using this model, which showed major biochemical changes in the granulosa cells at 12 and 48 h. The theca, however, was comparatively unaffected during atresia. The various studies using this model show changes in atretic follicles similar to those seen in other models and in the intact animal. The major advantage of this model is that it produces a large synchronous population of follicles that rapidly becomes atretic.
用30国际单位孕马血清促性腺激素（PMSG）预处理的切除垂体的雌性仓鼠，72小时后注射抗PMSG血清以诱导格拉夫卵泡闭锁。闭锁最早的组织学证据在4小时时可见于卵丘，核固缩显著增加。然而，壁颗粒细胞直到抗PMSG血清注射后12小时才出现核固缩。卵丘中核固缩数量最多在24小时时出现，此时卵母细胞发生假成熟。壁颗粒细胞的崩解在48小时时最为明显。对卵泡的形态测量显示，注射抗PMSG血清后48小时，颗粒层的厚度和面积显著下降。卵泡膜部分在4小时和12小时时的有丝分裂数量下降，在48小时时面积下降。然而，在注射抗PMSG血清后的72小时内未观察到卵泡膜细胞有核固缩。这些结果支持了此前使用该模型的研究工作，该研究表明颗粒细胞在12小时和48小时时发生了主要的生化变化。然而，卵泡膜在闭锁过程中相对未受影响。使用该模型的各种研究显示，闭锁卵泡的变化与其他模型以及完整动物中观察到的变化相似。该模型的主要优点是它能产生大量同步化的卵泡群体，这些卵泡会迅速发生闭锁。
