Groth D M, Wetherall J D, Carrick M J, Townend D, Lee I R
Anim Blood Groups Biochem Genet. 1985;16(3):193-203. doi: 10.1111/j.1365-2052.1985.tb01470.x.
Complement component C4 concentrations were measured in 40 pure bred Hereford cattle and 40 cattle from a mixed breed herd. Significant differences were not observed between the two groups studied nor between bulls and cows. However, the distribution of C4 concentrations was relatively disperse and appeared polymodal suggesting the presence of two isotypes of C4. Polyacrylamide gel electrophoresis of immunoprecipitated bovine C4 showed many samples to have two C4 alpha chains differing in relative molecular mass by about 1800. Isoelectric focusing of bovine plasma in agarose gels followed by immunofixation with specific anti-C4 antisera revealed two populations of native C4 differing in pI by about 0.3 pH unit. An association between the type of C4 alpha chain present and the pI of the native C4 molecule was observed. Collectively these findings indicate the presence of two structural C4 genetic loci in cattle.
对40头纯种赫里福德牛和40头混种牛群中的牛进行了补体成分C4浓度测定。在所研究的两组之间以及公牛和母牛之间均未观察到显著差异。然而，C4浓度的分布相对分散，呈现多峰分布，提示存在两种C4同种型。免疫沉淀牛C4的聚丙烯酰胺凝胶电泳显示，许多样品有两条相对分子质量相差约1800的C4α链。牛血浆在琼脂糖凝胶中进行等电聚焦，随后用特异性抗C4抗血清进行免疫固定，结果显示有两类天然C4，其等电点相差约0.3个pH单位。观察到存在的C4α链类型与天然C4分子的等电点之间存在关联。这些研究结果共同表明牛中存在两个结构C4基因位点。
