Li Xueying, Li Haixia, Wang Senlin, Zhang Huiping, Shao Yizhen, Chen Yun, Yuan Zhiliang
College of Life Science, Henan Agricultural University, Zhengzhou, China.
Baotianman National Nature Reserve of Hennan, Nanyang, China.
Appl Environ Microbiol. 2025 Aug 20;91(8):e0099225. doi: 10.1128/aem.00992-25. Epub 2025 Jul 30.
Based on global biotic homogenization, habitat generalists and specialists play an important role in maintaining the stability of ecosystems. However, limited information is available about the assembly processes and co-occurrence patterns of soil bacterial habitat specialists and generalists in forest ecosystems, particularly their response mechanisms to environmental factors. In this study, high-throughput sequencing technology was used to investigate the role of the ecological assemblage processes of soil bacterial habitat specialists and generalists and their role in maintaining the stability of the symbiotic network in temperate deciduous broad-leaved forests (China). The results showed that compared with specialists, the diversity of bacterial habitat generalists was lower, but their distribution ranges and environmental niche breadth were wider. Results from the null and neutral models indicate that, compared to deterministic processes, the community assembly of habitat generalists and specialists is more strongly influenced by stochastic processes, with generalists exhibiting a higher degree of stochasticity than specialists. Network analysis results showed that habitat specialists played a greater role in maintaining the stability of the bacterial co-occurrence network than the generalists. In addition, bacterial habitat specialists were more likely to be affected by light and spatial feature vectors than generalists. These findings provide a novel perspective for understanding the assembly processes and diversity maintenance mechanisms of the forest soil bacterial community.
Limited information is available about bacterial specialists and generalists in forests. Generalists were more affected by stochastic processes than specialists. Specialists played a more important role in network stability than generalists. Light and spatial vectors had stronger effects on specialists than generalists.
基于全球生物同质化，栖息地泛化种和特化种在维持生态系统稳定性方面发挥着重要作用。然而，关于森林生态系统中土壤细菌栖息地特化种和泛化种的组装过程和共现模式，尤其是它们对环境因素的响应机制，目前可用信息有限。在本研究中，利用高通量测序技术研究了土壤细菌栖息地特化种和泛化种的生态组装过程的作用及其在维持中国温带落叶阔叶林共生网络稳定性中的作用。结果表明，与特化种相比，细菌栖息地泛化种的多样性较低，但其分布范围和环境生态位宽度较宽。空模型和中性模型的结果表明，与确定性过程相比，栖息地泛化种和特化种的群落组装受随机过程的影响更大，泛化种表现出比特化种更高程度的随机性。网络分析结果表明，栖息地特化种在维持细菌共现网络稳定性方面比泛化种发挥了更大的作用。此外，细菌栖息地特化种比泛化种更容易受到光照和空间特征向量的影响。这些发现为理解森林土壤细菌群落的组装过程和多样性维持机制提供了新的视角。
关于森林中细菌特化种和泛化种的信息有限。泛化种比特化种更容易受到随机过程的影响。特化种在网络稳定性方面比泛化种发挥更重要的作用。光照和空间向量对特化种的影响比对泛化种的影响更强。
