Bulteau Rose, Barbier Lucie
Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.
Université Paris-Saclay, Univ Evry, CNRS, LAMBE, Evry-Courcouronnes, France.
Methods Mol Biol. 2025;2946:203-211. doi: 10.1007/978-1-0716-4658-8_16.
Oocyte quality is a major factor limiting female fertility. In the context of medically assisted reproduction (MAR), the assessment of oocyte quality is not yet well established, and researchers are screening for characteristics likely to influence pregnancy rate using a wide variety of approaches based on chemistry, -omics, optics, and physical parameters. In this context, microfluidics appears to be a promising technology as it allows the incorporation of multiple sensors on an easy-to-use platform, thus offering practical solutions for oocyte assessment in MAR routines. However, while a variety of microfluidic systems have been established for the manipulation of thousands or millions of cells, few are applicable to single cells of typical oocyte size, limiting the use of microfluidic devices in assisted reproductive technologies. We propose the design of a microfluidic device and a protocol for operating a single oocyte to any sensor integrated into the device and then retrieving it for fertilization. Our approach does not induce morphological alterations in oocytes and is compatible with standard MAR protocols.
卵母细胞质量是限制女性生育能力的主要因素。在医学辅助生殖（MAR）的背景下，卵母细胞质量的评估尚未完善，研究人员正在使用基于化学、组学、光学和物理参数的各种方法筛选可能影响妊娠率的特征。在这种情况下，微流控技术似乎是一项很有前景的技术，因为它允许在一个易于使用的平台上集成多个传感器，从而为MAR程序中的卵母细胞评估提供实用的解决方案。然而，虽然已经建立了各种用于操纵数千或数百万个细胞的微流控系统，但很少有适用于典型卵母细胞大小的单细胞的系统，这限制了微流控设备在辅助生殖技术中的应用。我们提出了一种微流控设备的设计方案以及一个操作流程，可将单个卵母细胞与集成在该设备中的任何传感器进行对接，然后将其取出用于受精。我们的方法不会引起卵母细胞的形态改变，并且与标准的MAR方案兼容。
