Zhang Chen, Peng Peiyao, Wen Jialong, Yuan Tongqi
State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing, 100083, PR China; Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, PR China.
Bioresour Technol. 2025 Nov;436:133048. doi: 10.1016/j.biortech.2025.133048. Epub 2025 Jul 28.
Enhancing the high-value utilization potential of lignocellulosic biomass through pretreatment fractionation has become a widely accepted strategy. In this study, a green and environmentally friendly deep eutectic solvent (DES) system was developed using Ru/C as a catalyst to enable the efficient fractionation and depolymerization of lignin, as well as to improve the saccharification efficiency of cellulose. Moso bamboo fibers and parenchyma cells, which possess distinct lignin structures and levels of biomass recalcitrance, were selected as feedstocks. The study focused on the influence of pretreatment temperature on lignin fractionation and depolymerization, as well as enzymatic saccharification of cellulose. The results revealed tissue-specific fractionation behaviors, with parenchyma cells exhibiting higher delignification ratio compared to fibers. Notably, the yield of lignin-derived monophenols from parenchyma cells reached up to 25.29%. Multidisciplinary characterization demonstrated significant structural differences in lignin fractions obtained from fibers and parenchyma cells, confirming the intrinsically lower recalcitrance of parenchyma cell walls. Subsequent enzymatic saccharification of the pretreated residues yielded glucose with a conversion efficiency as high as 93.19%. These findings suggest that this strategy holds great promise for advancing the practical application of lignocellulosic fractionation technologies and offers new insights into the valorization of structurally heterogeneous biomass resources.
通过预处理分馏提高木质纤维素生物质的高值利用潜力已成为一种广泛接受的策略。在本研究中，开发了一种绿色环保的低共熔溶剂（DES）体系，以Ru/C作为催化剂，实现木质素的高效分馏和解聚，并提高纤维素的糖化效率。选择具有不同木质素结构和生物质抗降解性水平的毛竹纤维和薄壁细胞作为原料。该研究重点关注预处理温度对木质素分馏和解聚以及纤维素酶糖化的影响。结果揭示了组织特异性的分馏行为，薄壁细胞的脱木质素率高于纤维。值得注意的是，薄壁细胞中木质素衍生单酚的产率高达25.29%。多学科表征表明，从纤维和薄壁细胞中获得的木质素组分存在显著的结构差异，证实了薄壁细胞壁本质上较低的抗降解性。预处理残渣随后的酶促糖化产生了转化率高达93.19%的葡萄糖。这些发现表明，该策略在推进木质纤维素分馏技术的实际应用方面具有巨大潜力，并为结构异质生物质资源的价值化提供了新的见解。
