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[生长中的幼龄公牛消化道中的蛋白质和氨基酸代谢。1. 以2,6-二氨基庚二酸为标记物测定十二指肠中细菌粗蛋白的流量]

[Protein and amino acid metabolism in the digestive tract growing young bulls. 1. Flow of bacterial raw protein in the duodenum determined by 2,6-diaminopimelic acid as a marker].

作者信息

Gabel M, Poppe S

出版信息

Arch Tierernahr. 1985 Aug;35(8):571-97. doi: 10.1080/17450398509425221.

Abstract

The influence of the composition of the rations on the content of diamino pimelic acid (DAP) and N in the bacteria dry matter and on the flow of bacteria crude protein into the duodenum, determined with DAP as marker, was determined on the basis of experimental investigations with young bulls provided with duodenal cannulae and with 28 different rations whose details are described. At a production level corresponding to dry matter intake and a variation range of the crude fibre content of between 11.3 and 29.1% in the DM, a content of N-free extractives between 50.9 and 77.4% in the DM, a content of soluble carbohydrates ibetween 5.3 and 6.4% in the DM, a crude protein content of between 6.4 and 17.1% in the DM and a pure protein content of between 4.9 and 15.5% in the DM, the DAP content of the bacteria DM amounts the 0.350 g 100 g DM +/- 0.090 the N-content of the bacteria DM amounts to 7.37 +/- 1.08 g/100 g DM, there are no relations between DAP- and N-content in the bacteria DM and the content of the individual carbohydrate fractions of the ration, there are positive relations between DAP- and N-content of the bacteria DM, the flow of bacterial crude protein into the duodenum amounts to 133 +/- 14 g/kg truly fermentable organic matter or 130 +/- 14 g/kg apparently digestible organic matter, there is a negative relation between bacteria crude protein at the duodenum (BCPD)/kg truly fermentable organic matter and the crude fibre content of the ration, there is a positive relation between BCPD/kg truly fermentable organic matter and N-free extractives and soluble carbohydrate content as well as the digestibility of the organic matter of the ration, there is no specific influence of the flow rate (kg digesta/kg intake of org. matter) or the dilution rate (g bacteriafree org. matter D/kg LW075/b) on BCPD/kg truly fermentable org. matter, there is a dependence of BCPD/kg truly fermentable org. matter on crude protein concentration in the ration in the concentration range of 6.4-9.0% crude protein in the ration (provided endogenous CP equivalents are used).

摘要

基于对安装了十二指肠套管的幼公牛进行的实验研究,以及28种不同日粮(其详细信息已作描述),测定了日粮组成对细菌干物质中二氨基庚二酸(DAP)和氮含量,以及以DAP为标记物测定的细菌粗蛋白流入十二指肠情况的影响。在对应于干物质摄入量的生产水平下,日粮中粗纤维含量在干物质中的变化范围为11.3%至29.1%,无氮浸出物含量在干物质中为50.9%至77.4%,可溶性碳水化合物含量在干物质中为5.3%至6.4%,粗蛋白含量在干物质中为6.4%至17.1%,纯蛋白含量在干物质中为4.9%至15.5%,细菌干物质的DAP含量为0.350 g/100 g干物质±0.090,细菌干物质的氮含量为7.37±1.08 g/100 g干物质,细菌干物质中的DAP和氮含量与日粮中各碳水化合物组分的含量之间无关联,细菌干物质中的DAP和氮含量之间存在正相关关系,细菌粗蛋白流入十二指肠的量为133±14 g/kg真可发酵有机物或130±14 g/kg表观可消化有机物,十二指肠中细菌粗蛋白(BCPD)/kg真可发酵有机物与日粮粗纤维含量之间存在负相关关系,BCPD/kg真可发酵有机物与无氮浸出物、可溶性碳水化合物含量以及日粮有机物消化率之间存在正相关关系,流速(kg食糜/kg有机物摄入量)或稀释率(g无细菌有机物D/kg LW0.75/b)对BCPD/kg真可发酵有机物无特定影响,在日粮粗蛋白浓度为6.4%至9.0%的范围内(假设使用内源性CP当量),BCPD/kg真可发酵有机物依赖于日粮中的粗蛋白浓度。

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