Bohórquez-Ávila Sonia P, Álvarez-Correa Diana C, Madroñero Johana, Velandia-Romero Myriam L, Castellanos Jaime E
Universidad El Bosque, Vicerrectoría de Investigaciones, Grupo de Virología, Bogotá, Colombia.
Universidad Nacional de Colombia, Facultad de Odontología, Grupo de Investigación Básica y Aplicada en Odontología, Bogotá, Colombia.
Acta Odontol Latinoam. 2025 Apr;38(1):29-38. doi: 10.54589/aol.38/1/29.
UNLABELLED: Human Cytomegalovirus (HCMV) is a major viral pathogen that causes severe complications in immunosuppressed individuals, particularly hematopoietic stem cell transplant recipients. In these patients, Cytomegalovirus has been associated with gastroenteritis, pneumonia, hepatitis, and even graft-versus-host disease, and a possible relationship has been identified between Cytomegalovirus genotypes and clinical course, complications and outcome. Early detection of Cytomegalovirus infection or reactivation is important, and previous findings show that it could potentially be evaluated in saliva, where HCMV causes asymptomatic viral shedding. Since saliva can be collected easily and safely, it is important to evaluate its potential for HCMV detection and genotyping, especially in pediatric patients who are receiving hematopoietic stem cell transplantation. AIM: The purpose of this study was to evaluate the feasibility of using saliva to detect and genotype HCMV in a cohort of pediatric hematopoietic stem cell transplant recipients (HSCTR). MATERIALS AND METHOD: This study was conducted at Fundación Hospital Pediátrico la Misericordia, in Bogota, Colombia. Stimulated saliva samples were collected once a week and subjected to HCMV detection by qualitative PCR and genotyping by nested PCR followed by sequencing. Finally, a phylogenetic tree was constructed. RESULTS: Twenty patients were enrolled, and 105 saliva samples were collected, of which 29 were positive for HCMV. Twelve patients had at least one positive sample. The gB1 genotype was identified with no coinfection with any other genotype. Phylogenetic analysis showed that some saliva samples were closer to the sequence reported for the Towne laboratory strain, while others were closer to the Merlin strain, with slight differences between them. CONCLUSIONS: It was demonstrated that saliva can be used to detect and genotype Cytomegalovirus in pediatric transplant recipients, and that sample collection is easy, with no risk of bleeding or discomfort in the pediatric patients evaluated.
未标记：人巨细胞病毒（HCMV）是一种主要的病毒病原体，可在免疫抑制个体，特别是造血干细胞移植受者中引起严重并发症。在这些患者中，巨细胞病毒与肠胃炎、肺炎、肝炎甚至移植物抗宿主病有关，并且已确定巨细胞病毒基因型与临床病程、并发症及结局之间可能存在关联。早期检测巨细胞病毒感染或再激活很重要，先前的研究结果表明，在HCMV导致无症状病毒脱落的唾液中可能对其进行评估。由于唾液采集简便安全，因此评估其在检测HCMV及基因分型方面的潜力很重要，尤其是在接受造血干细胞移植的儿科患者中。 目的：本研究旨在评估在一组儿科造血干细胞移植受者（HSCTR）中使用唾液检测HCMV并进行基因分型的可行性。 材料与方法：本研究在哥伦比亚波哥大的慈善儿科医院进行。每周采集一次刺激唾液样本，通过定性PCR检测HCMV，并通过巢式PCR随后测序进行基因分型。最后构建系统发育树。 结果：纳入20例患者，采集了105份唾液样本，其中29份HCMV呈阳性。12例患者至少有一份阳性样本。鉴定出gB1基因型，未与任何其他基因型合并感染。系统发育分析表明，一些唾液样本与汤氏实验室菌株报告的序列更接近，而其他样本与梅林菌株更接近，它们之间存在细微差异。 结论：证明唾液可用于检测儿科移植受者中的巨细胞病毒并进行基因分型，且样本采集简便，在所评估的儿科患者中无出血风险或不适。
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