Kara İkbal Hesna, Yildiz Esra, Meredova Guncha, Aksoy Hüseyin
Department of Biology, Faculty of Sciences, Sakarya University, Sakarya, Turkey.
Department of Biology, Faculty of Art and Sciences, Düzce University, Düzce, Turkey.
J Biochem Mol Toxicol. 2025 Aug;39(8):e70417. doi: 10.1002/jbt.70417.
Phenoxyethanol is widely used as a preservative in many cosmetic products and is considered to be safe. although there are some toxicity studies on phenoxyethanol, no detailed research evaluating its genotoxicity in human lymphocytes is available in the literature. This study aims to assess phenoxyethanol's cytotoxic and genotoxic profiles in human peripheral blood lymphocytes. For evaluation of genotoxic and cytotoxic effects of phenoxyethanol, chromosomal aberration (CA) and micronucleus (MN) tests were applied In both tests, lymphocyte cultures were treated with three concentrations (50, 25, 12.5 μg/mL) of phenoxyethanol and control groups (solvent, positive and negative controls). When examining the results of the chromosomal aberration test (CA) and the micronucleus test (MN), no significant differences were observed between the solvent control group and the application concentrations in either test. For the evaluation of cytotoxicity, mitotic index results showed a significant decrease at higher doses (50 and 25 μg/mL) compared to the solvent control. In conclusion, phenoxyethanol did not exhibit genotoxic effects (clastogenic and aneugenic) on human peripheral lymphocyte cells at the applied doses, although it did show a slight cytotoxic effect.
苯氧乙醇在许多化妆品中被广泛用作防腐剂，并且被认为是安全的。尽管有一些关于苯氧乙醇的毒性研究，但文献中没有详细评估其对人类淋巴细胞遗传毒性的研究。本研究旨在评估苯氧乙醇对人外周血淋巴细胞的细胞毒性和遗传毒性特征。为了评估苯氧乙醇的遗传毒性和细胞毒性作用，应用了染色体畸变（CA）和微核（MN）试验。在这两个试验中，淋巴细胞培养物用三种浓度（50、25、12.5μg/mL）的苯氧乙醇以及对照组（溶剂、阳性和阴性对照）进行处理。在检查染色体畸变试验（CA）和微核试验（MN）的结果时，在任一试验中，溶剂对照组与应用浓度之间均未观察到显著差异。对于细胞毒性评估，有丝分裂指数结果显示，与溶剂对照相比，较高剂量（50和25μg/mL）下有显著下降。总之，在所应用的剂量下，苯氧乙醇对人外周淋巴细胞未表现出遗传毒性作用（致断裂和致非整倍体作用），尽管它确实表现出轻微的细胞毒性作用。
