Sher Edward, Ko Meng-Wei, Kaur Kawaljit, Jewett Anahid
Division of Oral Biology and Medicine, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, University of California School of Dentistry, 10833 Le Conte Ave, 90095 Los Angeles, USA.
Division of Oral Biology and Medicine, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, University of California School of Dentistry, 10833 Le Conte Ave, 90095 Los Angeles, CA, USA.
Crit Rev Immunol. 2025;45(4):31-42. doi: 10.1615/CritRevImmunol.2025056318.
In this paper, we compared the ability to expand/supercharge NK cells (sNK) by either processed osteoclasts (pOCs) or live osteoclasts (OCs). pOCs induced similar levels of cell expansion in NK cells compared to live OCs. pOC-generated sNK cells exhibited lower lysis of oral squamous carcinoma stem-like cells (OSCSCs) tumors in comparison to live OC-generated sNK cells. Slightly lower levels of IFN-γ secretion were also observed in pOC-generated sNK cells when compared to live OC-generated sNK cells. Cytotoxic function and secretion levels of IFN-γ remained low in pOC-sNK cultures compared to OC-sNK cultures even after adding the supernatants harvested from live OCs to pOC-sNK cultures. pOCs were equally capable of selecting CD8+ T cells in sNK cell cultures when compared to live OCs. Overall, even though live OCs are capable of activating slightly better than the processed osteoclasts, the use of pOCs is preferable for the expansion of sNK cells due to shorter NK expansion period, higher cost-effectiveness, and faster availability for patient infusion.
在本文中，我们比较了经处理的破骨细胞（pOCs）或活破骨细胞（OCs）扩增/增强自然杀伤细胞（sNK）的能力。与活OCs相比，pOCs诱导NK细胞的细胞扩增水平相似。与活OCs产生的sNK细胞相比，pOC产生的sNK细胞对口腔鳞状癌干细胞样细胞（OSCSCs）肿瘤的杀伤作用较低。与活OCs产生的sNK细胞相比，在pOC产生的sNK细胞中也观察到较低水平的干扰素-γ分泌。即使将从活OCs收获的上清液添加到pOC-sNK培养物中，与OC-sNK培养物相比，pOC-sNK培养物中的细胞毒性功能和干扰素-γ分泌水平仍然较低。与活OCs相比，pOCs在sNK细胞培养物中同样能够筛选出CD8+ T细胞。总体而言，尽管活OCs的激活能力略优于经处理的破骨细胞，但由于NK扩增周期较短、成本效益较高且患者输注的可用性更快，使用pOCs进行sNK细胞扩增更为可取。
