Gaschik Tara, Eßbach Claudia, Fischer Dirk, Nickel Daniela, Ritz Ulrike
Department of Orthopaedics and Traumatology, University Medical Center, Johannes Gutenberg University, Langenbeckstraße 1, 55131 Mainz, Germany.
Duale Hochschule Sachsen - Staatliche Studienakademie Glauchau, University of Cooperative Education, Kopernikusstraße 51, 08371 Glauchau, Germany.
Biomater Adv. 2025 Dec;177:214422. doi: 10.1016/j.bioadv.2025.214422. Epub 2025 Jul 25.
In regenerative medicine, the demand for biomaterials with customizable properties to address diverse clinical challenges is steadily increasing. Collagen-based scaffolds offer significant promise for tissue engineering applications. This study presents a novel 5-layer collagen laminate engineered to facilitate both infection control and bone and tissue regeneration in open bone fractures. The laminate employs a layering strategy with rose bengal and green light-induced crosslinking to facilitate assembly while enabling the controlled release of three bioactive molecules, vancomycin, Bone morphogenetic protein 2 (BMP-2) and Stromal cell-derived factor 1 (SDF-1α). Mechanical properties were evaluated using a high-capacity load cell, revealing that multi-layer configurations exhibited reduced stiffness and tensile strength compared to single-layer laminates. Notably, incubation with Normal Human Dermal Fibroblasts (NHDF) holds the potential to enhance interlayer cohesion and improve the mechanical integrity of 5-layer laminates. Furthermore, the composition of collagen within the laminate played a critical role in determining both mechanical behavior and release kinetics. Singular sheets of Endoform™ Natural (E) collagen displayed rapid release, while Geistlich Bio-Gide® (G) collagen sheets provided sustained release, reflecting their distinct structural characteristics. These findings underscore the potential of multi-layer collagen laminates as a versatile platform for tailored therapeutic applications in regenerative medicine.
在再生医学中，对具有可定制特性以应对各种临床挑战的生物材料的需求正在稳步增长。基于胶原蛋白的支架在组织工程应用中具有巨大潜力。本研究提出了一种新型的5层胶原蛋白层压板，旨在促进开放性骨折中的感染控制以及骨骼和组织再生。该层压板采用了孟加拉玫瑰红和绿光诱导交联的分层策略，以促进组装，同时实现三种生物活性分子（万古霉素、骨形态发生蛋白2（BMP-2）和基质细胞衍生因子1（SDF-1α））的控释。使用高容量称重传感器评估了机械性能，结果表明，与单层层压板相比，多层结构的刚度和拉伸强度有所降低。值得注意的是，与正常人皮肤成纤维细胞（NHDF）一起孵育可能会增强层间凝聚力并改善5层层压板的机械完整性。此外，层压板中胶原蛋白的组成在决定机械行为和释放动力学方面起着关键作用。Endoform™ Natural（E）胶原蛋白的单层片显示出快速释放，而Geistlich Bio-Gide®（G）胶原蛋白片则提供持续释放，这反映了它们不同的结构特征。这些发现强调了多层胶原蛋白层压板作为再生医学中定制治疗应用的通用平台的潜力。
