Cucinello-Ragland Jessica A, Campos-Jurado Yolanda, Hershfelt Lila, Pujol Mateo, Saad Youssef, Zahoor Bilal, Neptune Alexandre, Morón Jose A
Department of Anesthesiology, Washington University in St. Louis, St. Louis, MO, USA; Pain Center, Washington University in St. Louis, St. Louis, MO, USA; School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
Department of Anesthesiology, Washington University in St. Louis, St. Louis, MO, USA.
Alcohol. 2025 Nov;128:21-27. doi: 10.1016/j.alcohol.2025.07.003. Epub 2025 Jul 29.
Chronic pain is a leading cause of disability, significantly decreases quality of life, and is highly co-morbid with substance use disorders, including alcohol use disorder (AUD). This is due, in part, to the pain-relieving effects of alcohol acting as a potential driving force for the progression and maintenance of AUD. Despite a substantial body of historic, anecdotal, clinical, and epidemiological evidence supporting the analgesic efficacy of alcohol, few preclinical studies have investigated the effects of pain on volitional alcohol drinking. Further, no studies to date have investigated aversion-resistant drinking in the context of persistent pain.
To address this gap in the literature, the current study combined quinine adulteration with the drinking in the dark (DID) model of binge-like alcohol drinking to assess the effects of complete Freund's adjuvant (CFA)-induced persistent inflammation on aversion-resistant binge-like alcohol drinking in female and male Long Evans rats.
Consistent with previous findings from our laboratory, CFA did not affect binge-like alcohol drinking in either sex, although female rats did consume greater levels of alcohol during baseline and post-CFA DID sessions. Similarly, CFA did not affect quinine adulterated binge-like alcohol drinking in either sex.
This study is the first to investigate the impact of persistent inflammation on aversion-resistant alcohol drinking. Although we found no effects of CFA on quinine adulterated binge-like alcohol drinking, these findings provide the groundwork for future investigations into this otherwise unstudied aspect of the pain-alcohol relationship.
慢性疼痛是导致残疾的主要原因，显著降低生活质量，且与包括酒精使用障碍（AUD）在内的物质使用障碍高度共病。这部分是由于酒精的止痛作用可能是AUD进展和维持的潜在驱动力。尽管有大量的历史、轶事、临床和流行病学证据支持酒精的镇痛效果，但很少有临床前研究调查疼痛对自愿饮酒的影响。此外，迄今为止，尚无研究在持续性疼痛的背景下调查抗厌恶饮酒情况。
为填补这一文献空白，本研究将奎宁掺假与类似暴饮的酒精饮用的黑暗中饮酒（DID）模型相结合，以评估完全弗氏佐剂（CFA）诱导的持续性炎症对雌性和雄性Long Evans大鼠抗厌恶类似暴饮的酒精饮用的影响。
与我们实验室之前的研究结果一致，CFA对两性的类似暴饮的酒精饮用均无影响，尽管雌性大鼠在基线和CFA后DID实验期间确实摄入了更高水平的酒精。同样，CFA对两性中掺有奎宁的类似暴饮的酒精饮用也没有影响。
本研究首次调查了持续性炎症对抗厌恶饮酒的影响。尽管我们发现CFA对掺有奎宁的类似暴饮的酒精饮用没有影响，但这些发现为未来对疼痛与酒精关系中这一尚未研究的方面进行调查奠定了基础。
