Güvenç Gizem, Erdoğan-Öner Aslı, Kavak Gülten, Akyol Bahçeci Selen, Kutlu Onur
Department of Oral and Maxillofacial Surgery, İzmir Katip Çelebi University, Faculty of Dentistry, İzmir, Turkey.
Department of Histology and Embryology, İzmir Katip Çelebi University, Faculty of Medicine, İzmir, Turkey.
J Oral Pathol Med. 2025 Sep;54(8):667-675. doi: 10.1111/jop.70018. Epub 2025 Jul 31.
Surgical procedures in oral and maxillofacial surgery inevitably cause wound formation, making the patient vulnerable to infections as well as discomfort. As antioxidant agents exert great potential to accelerate wound healing, we investigated the effects of N-acetylcysteine (NAC) and l-carnitine (LC) on palatal wound healing.
Sixty-four Wistar rats were randomly divided into four groups. 5 mm diameter wounds were created in the hard palates of the rats. 150 mg/kg/day NAC, 100 mg/kg/day LC, or both were injected intraperitoneally to the treatment groups until sacrification on Day 5 or 10. Hematoxylin and eosin, Masson's Trichrome staining, and immunohistochemistry (α-SMA, FGF-2) were performed on wound tissues.
On Days 5 and 10, reepithelialization was incomplete and inflammation was observed in all groups. Collagen density tended to increase in the LC and NAC + LC groups on Day 5, and significantly differed between the LC and NAC groups on day 10. A slight elevation in α-SMA expression was observed in the NAC group on Day 5, while NAC + LC treatment significantly reduced α-SMA levels compared to NAC alone. FGF-2 expression showed an increasing trend in the NAC + LC group on Day 5 and in the NAC and LC groups on Day 10. A significant increase in FGF-2 was observed in the NAC + LC group versus the NAC group on Day 5, and within the NAC group between Days 5 and 10.
Our findings have shown that LC in the late stage and NAC + LC combination in the early stage may positively affect palatal wound healing.
口腔颌面外科手术不可避免地会造成伤口，使患者易受感染并感到不适。由于抗氧化剂在加速伤口愈合方面具有巨大潜力，我们研究了N-乙酰半胱氨酸（NAC）和左旋肉碱（LC）对腭部伤口愈合的影响。
将64只Wistar大鼠随机分为四组。在大鼠的硬腭上制造直径5毫米的伤口。治疗组腹腔注射150毫克/千克/天的NAC、100毫克/千克/天的LC或两者，直至在第5天或第10天处死。对伤口组织进行苏木精和伊红染色、Masson三色染色以及免疫组织化学（α-SMA、FGF-2）检测。
在第5天和第10天，所有组的上皮再形成均不完全，且观察到炎症。在第5天，LC组和NAC + LC组的胶原密度有增加趋势，在第10天，LC组和NAC组之间有显著差异。在第5天，NAC组的α-SMA表达略有升高，而与单独使用NAC相比，NAC + LC治疗显著降低了α-SMA水平。在第5天，NAC + LC组的FGF-2表达呈上升趋势，在第10天，NAC组和LC组的FGF-2表达呈上升趋势。与NAC组相比，NAC + LC组在第5天FGF-2显著增加，在NAC组内第5天和第10天之间FGF-2也显著增加。
我们的研究结果表明，晚期的LC和早期的NAC + LC组合可能对腭部伤口愈合有积极影响。
