Babiak J, Nichols A V, Gong E L, McMahan C A, Kuehl T J, Mott G E, McGill H C
Atherosclerosis. 1985 Oct;57(1):1-17. doi: 10.1016/0021-9150(85)90133-9.
The effects of 2 different dietary fats (40% of calories from corn oil or coconut oil), in the presence of high-dietary cholesterol (1.7 mg/kcal), on the lipoprotein profiles of baboons (Papio cynocephalus sp) were studied by analytic ultracentrifugation, gradient gel electrophoresis (GGE), and heparin-manganese chloride precipitation. Relative to the corn oil (polyunsaturated fat) diet, the coconut oil (saturated fat) diet significantly increased total serum cholesterol by 43% (P less than 0.001) by increasing non-precipitable cholesterol (HDL-C) 58% (P less than 0.001) and precipitable cholesterol (VLDL + LDL-C) 35% (P less than 0.001). Analytic ultracentrifugal observations indicated that the increase in HDL-C was due to considerable increases in both HDL-I (baboon HDL of size 100-125 A and hydrated density 1.063-1.120 g/ml) and F1.20 degrees 9-28 lipoproteins (material of size 125-220 A and hydrated density 1.03-1.08 g/ml, and containing HDL apolipoproteins and apo E). Concentrations of other HDL subpopulations were unaffected by the dietary saturated rat. The increase in VLDL + LDL-C was due to increased LDL (S degree F 5-12 lipoproteins) and, to some extent, F1.20 degrees 9-28 lipoproteins because the larger, faster floating subspecies of the F1.20 degrees 9-28 lipoproteins were precipitable by heparin-manganese. In contrast, saturated fat (relative to polyunsaturated fat) induced lower concentrations of IDL (SF degree 12-20) and VLDL (SF degree 20-100). Lipoprotein size distributions by GGE indicated 5 HDL subpopulations and 2 or more LDL subpopulations in the sera of most baboons. The type of dietary fat did not affect the particle size range of each of the the HDL or LDL subpopulations. The results indicate that dietary fat markedly modulates the distribution of cholesterol between apo A-I-containing (HDL and F1.20 degrees 9-28) and apo B-containing (IDL and VLDL) lipoproteins without altering the presence of subpopulations based on particle size.
通过分析超速离心、梯度凝胶电泳（GGE）和肝素 - 氯化锰沉淀法，研究了在高膳食胆固醇（1.7毫克/千卡）存在的情况下，2种不同膳食脂肪（玉米油或椰子油提供40%的热量）对狒狒（Papio cynocephalus sp）脂蛋白谱的影响。相对于玉米油（多不饱和脂肪）饮食，椰子油（饱和脂肪）饮食通过使不可沉淀胆固醇（HDL - C）增加58%（P小于0.001）和可沉淀胆固醇（VLDL + LDL - C）增加35%（P小于0.001），使总血清胆固醇显著增加43%（P小于0.001）。分析超速离心观察表明，HDL - C的增加是由于HDL - I（大小为100 - 125 Å且水合密度为1.063 - 1.120克/毫升的狒狒HDL）和F1.20°9 - 28脂蛋白（大小为125 - 220 Å且水合密度为1.03 - 1.08克/毫升，含有HDL载脂蛋白和载脂蛋白E）均显著增加。其他HDL亚群的浓度不受膳食饱和脂肪比例的影响。VLDL + LDL - C的增加是由于LDL（S°F 5 - 12脂蛋白）增加，并且在一定程度上是由于F1.20°9 - 28脂蛋白增加，因为F1.20°9 - 28脂蛋白中较大、漂浮较快的亚类可被肝素 - 锰沉淀。相比之下，饱和脂肪（相对于多不饱和脂肪）导致IDL（SF°12 - 20）和VLDL（SF°20 - 100）的浓度较低。GGE分析的脂蛋白大小分布表明，大多数狒狒血清中有5个HDL亚群和2个或更多的LDL亚群。膳食脂肪的类型不影响每个HDL或LDL亚群的颗粒大小范围。结果表明，膳食脂肪显著调节含载脂蛋白A - I（HDL和F1.20°9 - 28）和含载脂蛋白B（IDL和VLDL）的脂蛋白之间胆固醇的分布，而不会改变基于颗粒大小的亚群的存在。
