Kwak Su-Hwan, Park Wonkeun John
Department of Natural and Life Sciences, Long Island University, 1 University Plaza, Brooklyn, NY, 11201, USA.
Crop Diversity & Improvement Laboratory, Pee Dee Research & Education Center, Clemson University, 2200 Pocket Road, Florence, SC, 29506, USA.
BMC Res Notes. 2025 Jul 31;18(1):340. doi: 10.1186/s13104-025-07345-6.
Plants control the direction of root growth toward moisture due to their sessile nature, a phenomenon known as hydrotropism. In Arabidopsis thaliana, the MIZU-KUSSEI1 (MIZ1) protein is essential for the hydrotropic response. To investigate the molecular mechanism of hydrotropism in cotton, we examined the function of GhMIZ1, the closest homolog of Arabidopsis thaliana MIZ1.
The GhMIZ1 gene, amplified from Gossypium hirsutum genomic DNA, was introduced into the Arabidopsis thaliana miz1 null mutant genome. Hydrotropism response in the transgenic mutant roots overexpressing GhMIZ1 was tested by using split-agarose plates containing 20% sorbitol. The expression of GhMIZ1 partially restored the hydrotropic response in miz1 mutant roots. This finding suggests that GhMIZ1 is capable of mediating hydrotropism in Arabidopsis thaliana, indicating that it serves as a functional homolog of MIZU-KUSSEI1.
由于植物固着生长的特性，它们能够控制根系朝着水分的方向生长，这种现象称为向水性。在拟南芥中，水向性应答蛋白1（MIZ1）对向水性反应至关重要。为了研究棉花向水性的分子机制，我们检测了拟南芥MIZ1最接近的同源物GhMIZ1的功能。
从陆地棉基因组DNA中扩增出GhMIZ1基因，并将其导入拟南芥miz1缺失突变体基因组中。通过使用含有20%山梨醇的裂层琼脂平板来测试过表达GhMIZ1的转基因突变体根的向水性反应。GhMIZ1的表达部分恢复了miz1突变体根的向水性反应。这一发现表明，GhMIZ1能够介导拟南芥的向水性，表明它作为水向性应答蛋白1的功能同源物。
