Kwon Hyuk Sung, Kim Geon Ha, Park Sun Ah, Moon So Young, Jang Jae-Won, Park Kee Hyung, Park Young Ho, Lim Jae-Sung, Yang Dong Won, Choi Seong Hye, Kim Byeong C, Kim SangYun, Koh Seong-Ho
Department of Neurology, Hanyang University Guri Hospital, Hanyang University College of Medicine, Guri, Korea.
Department of Neurology, Ewha Womans University Mokdong Hospital, Ewha Womans University College of Medicine, Seoul, Korea.
Dement Neurocogn Disord. 2025 Jul;24(3):162-173. doi: 10.12779/dnd.2025.24.3.162. Epub 2025 Jul 17.
Blood and cerebrospinal fluid (CSF) biomarkers are essential tools for the rapid diagnosis and monitoring of neurodegenerative diseases like Alzheimer's disease (AD). However, even minor variations in sample collection and storage procedures can significantly impact biomarker measurements, emphasizing the importance of standardized operating procedures. This review discusses the main pre-analytical factors that influence biomarker stability, outlines the best practices for blood and CSF collection and storage, and extensively analyzes recent research findings to ensure optimal reproducibility in biomarker studies. It also discusses the future directions and recommendations for enhancing biomarker research methodologies, including advancements in automated processing and quality control measures.
血液和脑脊液（CSF）生物标志物是快速诊断和监测阿尔茨海默病（AD）等神经退行性疾病的重要工具。然而，即使样本采集和储存程序的微小变化也会显著影响生物标志物的测量，这凸显了标准化操作程序的重要性。本综述讨论了影响生物标志物稳定性的主要分析前因素，概述了血液和脑脊液采集与储存的最佳实践，并广泛分析了近期的研究结果，以确保生物标志物研究具有最佳的可重复性。它还讨论了增强生物标志物研究方法的未来方向和建议，包括自动化处理和质量控制措施的进展。
