Hendy Aisha, Hassan Nazly, El-Nady Jehan, Ahmed Amal S I, Abou Shahba Rabab Mohamed, Tamer Tamer M
Chemistry Department, Faculty of Science (Girls), Al-Azhar University Nasr City Cairo Egypt.
Composites and Nanostructured Materials Research Department, Advanced Technologies and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg El-Arab City, P.O. Box: 21934 Alexandria Egypt.
RSC Adv. 2025 Jul 31;15(33):27300-27310. doi: 10.1039/d5ra00852b. eCollection 2025 Jul 25.
This study investigates the modification of casein protein with cinnamaldehyde Schiff base bonding, aiming to enhance its biological properties. The functionalization process was validated through different analytical techniques, including Fourier Transform Infrared Spectroscopy (FTIR), which confirmed the formation of Schiff base linkages. Thermal Gravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM) were employed to assess the thermal stability and morphological changes of modified protein, respectively. Biological evaluations revealed that the cinnamaldehyde-functionalized casein exhibited significant antibacterial activity against four different bacteria strains [two Gram-positive ( and ) and two Gram-negative ( and )], showcasing its potential as an antimicrobial agent. Antioxidant tests demonstrated enhanced scavenging abilities, suggesting protective effects against oxidative stress. Furthermore, preliminary hemocompatibility assessments indicated that the modified protein is non-toxic to human blood cells, underscoring its suitability for biomedical applications. Collectively, these findings suggest that cinnamaldehyde-functionalized casein holds promise for advancing healthcare materials.
本研究调查了用肉桂醛席夫碱键合修饰酪蛋白，旨在增强其生物学特性。通过不同的分析技术对功能化过程进行了验证，包括傅里叶变换红外光谱（FTIR），其证实了席夫碱键的形成。热重分析（TGA）和扫描电子显微镜（SEM）分别用于评估修饰蛋白的热稳定性和形态变化。生物学评估表明，肉桂醛功能化的酪蛋白对四种不同的细菌菌株[两种革兰氏阳性菌（ 和 ）和两种革兰氏阴性菌（ 和 ）]表现出显著的抗菌活性，展示了其作为抗菌剂的潜力。抗氧化测试表明其清除能力增强，表明对氧化应激具有保护作用。此外，初步的血液相容性评估表明，修饰后的蛋白对人体血细胞无毒，突出了其在生物医学应用中的适用性。总的来说，这些发现表明肉桂醛功能化的酪蛋白在推进医疗保健材料方面具有前景。
