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[用散射光研究水溶液中来自假结核耶尔森菌的脂多糖-蛋白质复合物]

[Study of a lipopolysaccharide-protein complex from Yersinia pseudotuberculosis in aqueous solutions by light scattering].

作者信息

Vasil'ev B K, Shekhovtsova M F, Ermak I M, Gladkikh R V, Solov'eva T F

出版信息

Biofizika. 1985 Nov-Dec;30(6):981-4.

PMID:4074765
Abstract

Lipopolysaccharide-protein complex isolated from Yersinia pseudotuberculosis forms aggregates in aqueous solutions. Dissociation of aggregates was found by light scattering method for 1 M sodium chloride, 1 M guanidine chloride, and urea solutions. Very large particles were detected also in these solutions and acid media. Little light scattering alterations have been observed for 0.27 g/l solution, and pronounced effect of temperature has been detected for more diluted solutions.

摘要

从假结核耶尔森氏菌中分离出的脂多糖-蛋白质复合物在水溶液中形成聚集体。通过光散射法发现,在1 M氯化钠、1 M氯化胍和尿素溶液中聚集体会发生解离。在这些溶液和酸性介质中也检测到了非常大的颗粒。对于0.27 g/l的溶液,观察到光散射变化很小,而对于更稀的溶液,则检测到温度有显著影响。

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