Ashwini M, Antony Ajit, Shilpa H S, Ravi Mangu S V V S, Sharan Kunal, Kapoor Mukesh
Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, U.P, India.
J Diet Suppl. 2025;22(5):750-774. doi: 10.1080/19390211.2025.2533889. Epub 2025 Aug 1.
Gut dysbiosis is a key feature in ulcerative colitis, a globally prevalent subtype of inflammatory bowel disease. Microbiota-directed dietary supplements can restore the dysbiotic gut and alleviate ulcerative colitis. We aimed to delineate whether prebiotic guar gum β-manno-oligosaccharides [GG-β-MOS(i) 500 mg/kg, (ii) 1000 mg/kg] favors gut microflora and microflora associated characteristics (MACs) and can prevent dextran sodium sulfate (DSS) induced ulcerative colitis in mice model. DSS challenged adult male C57BL/6 mice were used as model for colon inflammation evaluation. Food and water consumption, disease activity index (DAI), histopathological changes, cytokines in sera and colonic tissues, microflora associated characteristics (MACs; post-fermentation metabolites, short-chain fatty acids, etc.) and gut microflora profiles were studied. We found that GG-β-MOS successfully attenuated colonic inflammation, reduced inflammatory proteins, improved the ratio of Firmicutes to Bacteroidetes and promoted the colonization of probiotics like and . Furthermore, GG-β-MOS enriched beneficial bacteria , , , and showed positive correlation with anti-inflammatory metabolites like D-mannose, coprostanol, pentadecane, alpha-linolenic acid, dihomo-γ-linolenic acid, D-ribose, L-fucose, D-arabinose, N-acetyl-D-glucosamine, acetate and butyrate and negative correlation with oxalate, palmitic acid, arachidonic acid and erythritol. Overall, GG-β-MOS supplementation restored altered gut microflora and metabolome leading to improved mucosal repair and reduced intestinal inflammation in DSS induced ulcerative colitis mice model.
肠道微生物群失调是溃疡性结肠炎的一个关键特征，溃疡性结肠炎是一种全球流行的炎症性肠病亚型。针对微生物群的膳食补充剂可以恢复失调的肠道并缓解溃疡性结肠炎。我们旨在确定益生元瓜尔胶β-甘露寡糖[GG-β-MOS（i）500毫克/千克，（ii）1000毫克/千克]是否有利于肠道微生物群和微生物群相关特征（MACs），并能否在小鼠模型中预防葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎。用DSS攻击的成年雄性C57BL/6小鼠作为结肠炎症评估模型。研究了食物和水的消耗量、疾病活动指数（DAI）、组织病理学变化、血清和结肠组织中的细胞因子、微生物群相关特征（MACs；发酵后代谢产物、短链脂肪酸等）和肠道微生物群谱。我们发现，GG-β-MOS成功减轻了结肠炎症，减少了炎症蛋白，改善了厚壁菌门与拟杆菌门的比例，并促进了如[具体益生菌名称1]和[具体益生菌名称2]等益生菌的定殖。此外，GG-β-MOS富集的有益细菌[具体有益细菌名称1]、[具体有益细菌名称2]、[具体有益细菌名称3]、[具体有益细菌名称4]和[具体有益细菌名称5]与抗炎代谢产物如D-甘露糖、粪甾烷醇、十五烷、α-亚麻酸、二高-γ-亚麻酸、D-核糖、L-岩藻糖、D-阿拉伯糖、N-乙酰-D-葡萄糖胺、乙酸盐和丁酸盐呈正相关，并与草酸盐、棕榈酸、花生四烯酸和赤藓糖醇呈负相关。总体而言，补充GG-β-MOS可恢复改变的肠道微生物群和代谢组，从而改善DSS诱导的溃疡性结肠炎小鼠模型的黏膜修复并减轻肠道炎症。
