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[通过吸附和离子交换色谱法对人可溶性白细胞抗原进行制备性分级分离]

[Preparative fractionation of human soluble leukocyte antigens by means of adsorption and ion-exchange chromatography].

作者信息

Petrunin D D, Lopukhin Iu M, Olefirenko G A

出版信息

Biull Eksp Biol Med. 1985 Dec;100(12):730-1.

PMID:4074879
Abstract

With monospecific test-system control, preparative fractionation of 9 individual soluble human leucocyte antigens has been carried out using adsorption chromatography with silicagel, kaolin, Al2O3, Ba2SO3 and chromatography with ion-exchange Sephadexes. Optimal conditions for the preparation of purified leucocyte antigens were determined.

摘要

采用单特异性检测系统对照,使用硅胶、高岭土、Al2O3、Ba2SO3进行吸附色谱法以及离子交换葡聚糖凝胶色谱法,对9种个体可溶性人类白细胞抗原进行了制备性分级分离。确定了制备纯化白细胞抗原的最佳条件。

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