GATA3 regulates Th1/Th2 balance in allergic rhinitis by interacting with RUNX1.

OBJECTIVE

To investigate the action mechanism of GATA3 and Runt-related transcription factor 1(RUNX1) in modulating regulatory the Th1/Th2 cell differentiation and treat allergic rhinitis (AR).

METHODS

Established AR model mice by ovalbumin (OVA) and isolated the peripheral blood monocytes (PBMCs), evaluating behavioral changes of AR mice. Analysis of histopathological characteristics by HE. sIgE, IL-4, and IFN-γ were detected by ELISA. RT-qPCR and Western blot detected the expression of GATA3, T-bet, and RUNX1. Using immunofluorescence to detect the expression of GATA and T-bet.

RESULTS

Knockout GATA3 suppressed the expression of GATA3 and concentration of sIgE and IL-4, and promoted the expression of T-bet and concentration of IFN-γ. Knockout GATA3 alleviates inflammation in AR mice by increasing the CD4 IFN-γ cell rate and decreasing the CD4 IL-4 cell rate. RUNX1 and GATA3 were both expressed in the nucleus, GATA3 could bind and interact with RUNX1, si-RUNX1 reversed the effect of sh-GATA3, RUNX1 regulated the balance of Th1/Th2 to alleviate inflammation in AR mice by down-regulating expression of GATA3.

CONCLUSION

GATA3 regulated the balance of Th1/Th2 cells maybe by the interaction between RUNX1 to improve anti-allergic and anti-inflammatory potential strategies in AR therapy.