Yang Fengqi, Xiao Dandan, Kim Young-Sang, Nagahawatta D P, Lee Hyo-Geun, Kim Daekyung, Jeon You-Jin
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea.
Int J Biol Macromol. 2025 Sep;321(Pt 3):146492. doi: 10.1016/j.ijbiomac.2025.146492. Epub 2025 Aug 2.
Inflammatory bowel disease (IBD), characterized by chronic intestinal inflammation and compromised gut barrier integrity, remains a significant challenge in clinical management. This study explored the synergistic potential of Sargassum horneri polysaccharides (SHCPs) and Lactobacillus plantarum (LAB) in alleviating DSS-induced colitis. SHCPs were characterized using FTIR and SEM in this study, and their structural features-including high sulfate content, molecular weight distribution, and glycosidic linkages-were previously elucidated in our earlier work, supporting their classification as bioactive sulfate-rich polysaccharides and suggesting structure-dependent functionality. In vitro experiments using Caco-2 cell models showed that SHCPs promoted LAB adhesion and, in combination, significantly reduced pro-inflammatory cytokine levels (IL-6, IL-1β, TNF-α, and PGE2), thereby mitigating DSS-induced inflammation. This effect correlated with improved microbiota-host interactions and was linked to restoration of tight junction proteins (ZO-1, Occludin), suppression of Claudin-2, and downregulation of iNOS and COX-2. In IBD-induced zebrafish models, the synergistic effects of SHCPs and LAB were further validated, as evidenced by enhanced gut barrier integrity, alleviation of dysbiosis, and restoration of intestinal length. These findings highlight the potential synergistic effects of SHCPs and LAB as a combinatorial strategy to regulate gut inflammation and enhancing epithelial barrier function, potentially offering new insights and therapeutic opportunities for IBD intervention.
炎症性肠病（IBD）以慢性肠道炎症和肠道屏障完整性受损为特征，在临床管理中仍然是一个重大挑战。本研究探讨了羊栖菜多糖（SHCPs）和植物乳杆菌（LAB）在减轻葡聚糖硫酸钠（DSS）诱导的结肠炎方面的协同潜力。在本研究中，使用傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）对SHCPs进行了表征，其结构特征，包括高硫酸盐含量、分子量分布和糖苷键，在我们早期的工作中已经阐明，支持将其分类为富含生物活性硫酸盐的多糖，并表明其功能依赖于结构。使用Caco-2细胞模型进行的体外实验表明，SHCPs促进了LAB的黏附，并且联合使用时显著降低了促炎细胞因子水平（IL-6、IL-1β、TNF-α和PGE2），从而减轻了DSS诱导的炎症。这种作用与改善微生物群与宿主的相互作用相关，并与紧密连接蛋白（ZO-1、闭合蛋白）的恢复、Claudin-2的抑制以及诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的下调有关。在IBD诱导的斑马鱼模型中，SHCPs和LAB的协同作用得到了进一步验证，表现为肠道屏障完整性增强、菌群失调减轻和肠道长度恢复。这些发现突出了SHCPs和LAB作为调节肠道炎症和增强上皮屏障功能的联合策略的潜在协同作用，可能为IBD干预提供新的见解和治疗机会。
