Awadalla Maaweya, Al Rawi Halah Z, Alahmadi Reham M, Khojah Osamah T, Al-Shouli Samia T, Almansour Mansour I, Alosaimi Bandar
Research Center, King Fahad Medical City, Riyadh Second Health Cluster, Riyadh, Saudi Arabia.
College of Science, King Saud University, Riyadh, Saudi Arabia.
Tumour Virus Res. 2025 Aug 5;20:200327. doi: 10.1016/j.tvr.2025.200327.
Cervical cancer is the fourth most common cancer among women globally, with a woman dying every 2 min. Despite the need to understand the tumor microenvironment (TME) transcriptome of cervical squamous cell carcinoma (CSCC) and cervical intraepithelial neoplasia grade 3 (CIN 3), studies remain limited. This study compares the TME transcriptome of HPV-positive CSCC and CIN 3, analyzing 168 genes involved in tumor cell interactions with inflammatory and immune mediators, transcription, signal transduction, oncogenesis, tumor suppression, angiogenesis, and apoptosis. Co-expressed genes identified in HPV + CSCC and CIN 3 were analyzed using computational biology. Gene Ontology and KEGG enrichment identified relevant biological pathways and cancer hallmarks. Fifty-five co-expressed genes were linked to cancer pathways, inflammatory responses, cell migration, and development. KEGG enrichment highlighted viral protein interactions involving cytokines, IL-17 signaling, and chemokine receptor interactions. These genes were associated with cancer hallmark pathways, including angiogenesis, inflammation, proliferation, genomic instability, invasion, and metastasis. Their similar expression in CSCC and CIN 3 suggests a potential prognostic value and that CIN 3 progression may involve changes in gene expression. We propose the term "CSCC-like carcinoma," indicating CIN 3's increased invasive potential at the molecular level.
宫颈癌是全球女性中第四大常见癌症，每两分钟就有一名女性死亡。尽管有必要了解宫颈鳞状细胞癌（CSCC）和宫颈上皮内瘤变3级（CIN 3）的肿瘤微环境（TME）转录组，但相关研究仍然有限。本研究比较了HPV阳性CSCC和CIN 3的TME转录组，分析了168个参与肿瘤细胞与炎症和免疫介质相互作用、转录、信号转导、肿瘤发生、肿瘤抑制、血管生成和凋亡的基因。使用计算生物学分析了在HPV + CSCC和CIN 3中鉴定出的共表达基因。基因本体论和KEGG富集分析确定了相关的生物学途径和癌症特征。55个共表达基因与癌症途径、炎症反应、细胞迁移和发育相关。KEGG富集分析突出了涉及细胞因子、IL-17信号传导和趋化因子受体相互作用的病毒蛋白相互作用。这些基因与癌症特征途径相关，包括血管生成、炎症、增殖、基因组不稳定性、侵袭和转移。它们在CSCC和CIN 3中的相似表达表明具有潜在的预后价值，并且CIN 3的进展可能涉及基因表达的变化。我们提出了“CSCC样癌”这一术语，表明CIN 3在分子水平上具有增加的侵袭潜力。
