Mohanan Arvind, Prashanthi S K, Arun Y P, Raghunandana A, Krishnaraj P U, Nayak Spurthi N, Hegde Yashoda
Department of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka, 580005, India.
Department of Plant Pathology, Sri Karan Narendra Agriculture University, Jobner, Jaipur, Rajasthan, 303329, India.
Sci Rep. 2025 Aug 2;15(1):28242. doi: 10.1038/s41598-025-85918-y.
Sheath blight (ShB) disease, caused by Rhizoctonia solani Kuhn, poses a significant economic threat to rice production world wide. Acknowledging the limited understanding of ShB resistance proteomics in highly resistant germplasm, our study aimed to unravel the proteomic intricacies underlying the interaction between resistant landrace Nizam Shait and R. solani. Utilizing Nizam Shait and BPT-5204 as representatives of ShB resistance and susceptibility, a comparative proteome analysis was performed using Orbitrap-Fusion mass spectrometry. The analysis unveiled 5133 differentially expressed proteins, with 118 significantly upregulated and 172 significantly downregulated at 0.05 p-value. Functional annotation revealed that the proteins associated with jasmonic acid-induced systemic resistance (JA-ISR), brassinosteroid (BR) signaling pathway, terpenoid biosynthesis, cell wall remodeling and carbohydrate metabolism showed significant upregulation in Nizam Shait upon ShB infection. The proteins associated with systemic acquired resistance (SAR), pathogenesis related proteins, cell redox homeostasis and cell death were downregulated, Notably, the 14-3-3 like protein GF-E exhibited highest upregulation, indicating its pivotal role in defense modulation through the brassinosteroid signaling pathway. The two key proteins of gamma-aminobutyric acid (GABA) shunt mediated defense, Succinate-semialdehyde dehydrogenase and Gamma-aminobutyrate transaminase were upregulated in Nizam Shait versus BPT-5204 and many other defense proteins were upregulated. Key signaling pathways involved in ShB resistance in Nizam Shait encompassed PTI via JA-ISR, cell wall strengthening, and brassinosteroid mediated resistance. Validation of the proteome data through RT-qPCR corroborated the findings, highlighting the significance of this research for future proteome assisted breeding efforts aimed at developing ShB resistant rice varieties.In conclusion, the current study deciphers pathways responsible for high resistance in landrace Nizam Shait against R. solani and identifies key proteins in Rice-R. solani interaction.
纹枯病（ShB）由立枯丝核菌库恩氏菌引起，对全球水稻生产构成重大经济威胁。鉴于对高抗种质中纹枯病抗性蛋白质组学的了解有限，我们的研究旨在揭示抗性地方品种尼扎姆·沙伊特与立枯丝核菌之间相互作用背后的蛋白质组学复杂性。以尼扎姆·沙伊特和BPT - 5204分别作为纹枯病抗性和易感性的代表，使用轨道阱融合质谱进行了比较蛋白质组分析。分析揭示了5133种差异表达蛋白，在p值为0.05时，有118种显著上调，172种显著下调。功能注释表明，与茉莉酸诱导的系统抗性（JA - ISR）、油菜素内酯（BR）信号通路、萜类生物合成、细胞壁重塑和碳水化合物代谢相关的蛋白质在纹枯病感染后在尼扎姆·沙伊特中显著上调。与系统获得性抗性（SAR）、病程相关蛋白、细胞氧化还原稳态和细胞死亡相关的蛋白质下调。值得注意的是，14 - 3 - 3样蛋白GF - E表现出最高上调，表明其在通过油菜素内酯信号通路进行防御调节中起关键作用。与γ-氨基丁酸（GABA）分流介导的防御相关的两个关键蛋白，琥珀酸半醛脱氢酶和γ-氨基丁酸转氨酶在尼扎姆·沙伊特中相对于BPT - 5204上调，许多其他防御蛋白也上调。尼扎姆·沙伊特中参与纹枯病抗性的关键信号通路包括通过JA - ISR的PTI、细胞壁强化和油菜素内酯介导的抗性。通过RT - qPCR对蛋白质组数据进行验证证实了这些发现，突出了本研究对于未来旨在培育抗纹枯病水稻品种的蛋白质组辅助育种工作的重要性。总之，当前研究解读了地方品种尼扎姆·沙伊特对立枯丝核菌高抗性的相关途径，并鉴定了水稻 - 立枯丝核菌相互作用中的关键蛋白。
