Akkaya Yüksel, Erdin Begüm Nalça, Yılmaz Ahmet Münir, Kılıç İbrahim Halil, Toraman Zülal Aşçı
From the Department of Medical Microbiology, Faculty of Medicine, University of Health Sciences Hamidiye, Istanbul, Türkiye.
From the Department of Medical Microbiology, Ümraniye Eğitim Ve Araştırma Hastanesi, Istanbul, Türkiye.
Ann Saudi Med. 2025 Jul-Aug;45(4):207-217. doi: 10.5144/0256-4947.2025.207. Epub 2025 Aug 7.
is a high priority fungal pathogen due to its antifungal resistance and its association with increased morbidity and mortality in infected patients.
The aim of this study was to identify Candida species in clinical samples and to determine the clades and in vitro antifungal resistance of .
Retrospective cohort.
Single-center tertiary hospital in Türkiye.
The study was conducted in the Medical Microbiology Laboratory of Ümraniye Training and Research Hospital between December 2023 and October 2024. Fungal samples were identified using bio-Mérieux VITEK MS v.3.2 (bio-Mérieux, France) and RT-PCR. Antifungal susceptibility testing of was performed by VITEK 2 Compact AST YS08 and SYO.
Identification of Candida species, in-vitro antifungal resistance of .
846 fungal isolates obtained from 746 patients were included.
A total of 846 fungal isolates were identified, with being the most common (n=440, 52%), followed by (n=124, 14.7%), (n=85, 10.1%), (n=69, 8.2%) and (n=57, 6.7%). All isolates were susceptible to anidulafungin. Of these isolates, 47 (82%) were resistant to fluconazole, 34 (60%) to amphotericin B, four (7%) to caspofungin and three (5%) to micafungin. One isolate was resistant to amphotericin B, fluconazole, caspofungin and micafungin. A total of 31 (54%) isolates were resistant to amphotericin B and fluconazole. In accordance with the manufacturer's recommendations, 57 isolates were evaluated as Clade-1.
infections are becoming increasingly common. In order to better understand antifungal-resistance of this pathogen, advanced methods should be used for rapid detection of clades and mutations in the FKS gene should be revealed.
Single center, whole genome sequence analysis were not performed.
由于其抗真菌耐药性以及与感染患者发病率和死亡率增加相关，是一种高度优先关注的真菌病原体。
本研究的目的是鉴定临床样本中的念珠菌属物种，并确定其进化枝以及体外抗真菌耐药性。
回顾性队列研究。
土耳其的一家单中心三级医院。
该研究于2023年12月至2024年10月在乌姆拉尼耶培训与研究医院的医学微生物实验室进行。使用生物梅里埃VITEK MS v.3.2（法国生物梅里埃公司）和逆转录聚合酶链反应（RT-PCR）鉴定真菌样本。通过VITEK 2 Compact AST YS08和SYO对其进行抗真菌药敏试验。
念珠菌属物种的鉴定，其体外抗真菌耐药性。
纳入了从746例患者中获得的846株真菌分离株。
共鉴定出846株真菌分离株，其中最常见的是（n = 440，52%），其次是（n = 124，14.7%）、（n = 85，10.1%）、（n = 69，8.2%）和（n = 57，6.7%）。所有分离株对阿尼芬净敏感。在这些分离株中，47株（82%）对氟康唑耐药，34株（60%）对两性霉素B耐药，4株（7%）对卡泊芬净耐药，3株（5%）对米卡芬净耐药。1株分离株对两性霉素B、氟康唑、卡泊芬净和米卡芬净均耐药。共有31株（54%）分离株对两性霉素B和氟康唑耐药。根据制造商的建议，57株分离株被评估为进化枝1。
感染越来越常见。为了更好地了解这种病原体的抗真菌耐药性，应使用先进方法快速检测进化枝，并揭示FKS基因中的突变。
单中心研究，未进行全基因组序列分析。
