从口腔细胞拭子测量线粒体呼吸能力的方案。

Protocol for measuring mitochondrial respiratory capacity from buccal cell swabs.

作者信息

Ram Tina R, Mu Chunlong, Campbell Jaclyn C, MacEachern Sarah J, Shearer Jane

机构信息

Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada.

Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.

出版信息

STAR Protoc. 2025 Aug 2;6(3):104004. doi: 10.1016/j.xpro.2025.104004.

DOI:10.1016/j.xpro.2025.104004

PMID:40753577

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12339751/

Abstract

Mitochondrial respirometry provides a detailed assessment of oxygen consumption within the electron transport system, yet methods detailing respiration from non-invasive samples remain limited. Here, we present a protocol for measuring mitochondrial respiration in cultured buccal cells. We outline procedures for buccal cell collection, primary cell culture, and respirometry calibration, followed by oxygen consumption measurements and cell count for data normalization. This protocol allows reliable evaluation of mitochondrial function from non-invasive buccal cell samples, offering a valuable tool for metabolic investigation.

摘要

线粒体呼吸测定法可详细评估电子传递系统中的氧气消耗情况，但针对非侵入性样本进行呼吸测定的详细方法仍然有限。在此，我们介绍一种用于测量培养的颊细胞中线粒体呼吸的方案。我们概述了颊细胞收集、原代细胞培养和呼吸测定校准的程序，随后进行氧气消耗测量和细胞计数以进行数据归一化。该方案能够从非侵入性颊细胞样本中可靠地评估线粒体功能，为代谢研究提供了一个有价值的工具。