Sun Yanhua, Xie Zhiyu, Shang Shuang, Zheng Meiwen, Jia Wenhao, Wang Xiaobo
College of Chemical and Materials Engineering, Xuchang University, 461000, PR China.
College of Chemical and Materials Engineering, Xuchang University, 461000, PR China; Collaborative Innovation Center of Functional Food by Green Manufacturing, Henan Province, Xuchang 461000, China.
J Chromatogr A. 2025 Sep 27;1759:466256. doi: 10.1016/j.chroma.2025.466256. Epub 2025 Jul 29.
Chrysin is a common flavonoid compound with various pharmacological activities, which often exists in natural dietary sources such as propolis and honey. To selectively isolate and enrich chrysin from propolis, porous flower-like molecularly imprinted polymers nanomaterials were developed using a facile synthesis approach. The material was constructed by first decorating UiO-66 onto N-doped porous carbon flowers (NCFs), followed by coating the surface with a thin imprinting layer, yielding NCFs@UiO-66@MIPs. The scaffold material possesses a high specific surface area, large pore volume, and wide average pore diameter, endowing the synthesized NCFs@UiO-66@MIPs with a considerable affinity for chrysin. The maximum equilibrium adsorption capacity reached 117.6 mg g, with binding behavior consistent with monolayer adsorption. Compared to its structural analogues kaempferol, daidzein, quercetin, and genistein, NCFs@UiO-66@MIPs demonstrates better recognition ability and recovery for chrysin, achieving an imprinting factor of 3.73. After 20 adsorption-desorption cycles, the material retained 79.2 % of its initial adsorption capacity. Furthermore, the synthesized NCFs@UiO-66@MIPs demonstrated excellent anti-interference properties and regenerability, enabling highly selective chrysin enrichment from propolis. This work provides a promising imprinting adsorbent for the targeted extraction of bioactive ingredient from propolis and other complex dietary matrix.
白杨素是一种具有多种药理活性的常见黄酮类化合物，常见于蜂胶和蜂蜜等天然饮食来源中。为了从蜂胶中选择性地分离和富集白杨素，采用简便的合成方法制备了多孔花状分子印迹聚合物纳米材料。该材料的构建过程是先将UiO-66修饰在氮掺杂多孔碳花（NCFs）上，然后在其表面涂覆一层薄的印迹层，得到NCFs@UiO-66@MIPs。支架材料具有高比表面积、大孔体积和较宽的平均孔径，使得合成的NCFs@UiO-66@MIPs对白杨素具有相当大的亲和力。最大平衡吸附容量达到117.6 mg/g，结合行为符合单层吸附。与结构类似物山奈酚、大豆苷元、槲皮素和染料木黄酮相比，NCFs@UiO-66@MIPs对白杨素表现出更好的识别能力和回收率，印迹因子达到3.73。经过20次吸附-解吸循环后，该材料仍保留其初始吸附容量的79.2%。此外，合成的NCFs@UiO-66@MIPs表现出优异的抗干扰性能和可再生性，能够从蜂胶中高度选择性地富集白杨素。这项工作为从蜂胶和其他复杂饮食基质中靶向提取生物活性成分提供了一种有前景的印迹吸附剂。
