Co-doped MIL-101 (Fe,Co) combined with molecularly imprinted: a fluorescence sensing strategy for rapid detection on sulfamethazine.

For the purpose of overcoming the problems of traditional molecularly imprinted Polymers(MIPs), which need to be combined with instrumental detection, complicated and inefficient operation, MIL-101(Fe,Co)@MIP fluorescent sensor was developed in this study for the rapid and accurate detection of sulfamethazine (SM2). In this study, a bimetallic Metal-Organic Frameworks(MOF) called MIL-101(Fe,Co) was prepared by Co doping into MIL-101(Fe), exhibiting 1.6-fold enhancement in peroxidase like activity compared to the MIL-101(Fe). Additionally, the integration of MIL-101(Fe,Co) and mixed monomer imprinted polymers brought the specific surface area augmentation by a factor approaching two, coupled with approximately 5-fold amplification in detection efficacy. Adsorption performance tests were conducted on MIL-101(Fe,Co)@MIP, achieving an adsorption capacity of 24.33 mg/g with the adsorption time reduced to 30 min. In this work, we developed a novel fluorescence sensor using MIL-101(Fe,Co)@MIP as a method for SM2 detection, and the method exhibited a linear detection range from 0.02 to 25 μM, with the detection limit as low as 6.5 μg/L while showing excellent interference resistance and stability. The method was also applied to real sample detection, showing an average recovery range of SM2 in water samples from 81.31 % to 97.11 % with a relative standard deviation (RSD) of 0.57 %-3.20 %, and no significant difference compared to the UPLC-MS/MS method, demonstrating good accuracy and practicality. All in all, the MIL-101(Fe,Co)@MIP fluorescence sensing detection method is easy to operate, fast in detection, and serves as a novel approach suitable for the rapid detection of SM2.