Liu Peiqiao, Yu Yongtong, Mo Huixin, Lin Jiena, Luo Lin, Wei Xiaoqun
Guangdong Provincial Key Laboratory of Food Quality and Safety/ National-Local Joint Engineering Research Center for Precision Machining and Safety of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Wuzhou Institute for Food and Drug Control, Wuzhou, 543000, China.
Talanta. 2025 Jul 28;297(Pt A):128628. doi: 10.1016/j.talanta.2025.128628.
For the purpose of overcoming the problems of traditional molecularly imprinted Polymers(MIPs), which need to be combined with instrumental detection, complicated and inefficient operation, MIL-101(Fe,Co)@MIP fluorescent sensor was developed in this study for the rapid and accurate detection of sulfamethazine (SM2). In this study, a bimetallic Metal-Organic Frameworks(MOF) called MIL-101(Fe,Co) was prepared by Co doping into MIL-101(Fe), exhibiting 1.6-fold enhancement in peroxidase like activity compared to the MIL-101(Fe). Additionally, the integration of MIL-101(Fe,Co) and mixed monomer imprinted polymers brought the specific surface area augmentation by a factor approaching two, coupled with approximately 5-fold amplification in detection efficacy. Adsorption performance tests were conducted on MIL-101(Fe,Co)@MIP, achieving an adsorption capacity of 24.33 mg/g with the adsorption time reduced to 30 min. In this work, we developed a novel fluorescence sensor using MIL-101(Fe,Co)@MIP as a method for SM2 detection, and the method exhibited a linear detection range from 0.02 to 25 μM, with the detection limit as low as 6.5 μg/L while showing excellent interference resistance and stability. The method was also applied to real sample detection, showing an average recovery range of SM2 in water samples from 81.31 % to 97.11 % with a relative standard deviation (RSD) of 0.57 %-3.20 %, and no significant difference compared to the UPLC-MS/MS method, demonstrating good accuracy and practicality. All in all, the MIL-101(Fe,Co)@MIP fluorescence sensing detection method is easy to operate, fast in detection, and serves as a novel approach suitable for the rapid detection of SM2.
为克服传统分子印迹聚合物（MIPs）需要与仪器检测相结合、操作复杂且效率低下的问题，本研究开发了一种MIL-101(Fe,Co)@MIP荧光传感器，用于快速、准确地检测磺胺二甲嘧啶（SM2）。在本研究中，通过将Co掺杂到MIL-101(Fe)中制备了一种双金属金属有机框架（MOF），即MIL-101(Fe,Co)，与MIL-101(Fe)相比，其类过氧化物酶活性提高了1.6倍。此外，MIL-101(Fe,Co)与混合单体印迹聚合物的结合使比表面积增加了近两倍，同时检测效率提高了约5倍。对MIL-101(Fe,Co)@MIP进行了吸附性能测试，吸附容量达到24.33 mg/g，吸附时间缩短至30分钟。在本工作中，我们开发了一种以MIL-101(Fe,Co)@MIP为检测SM2的新型荧光传感器方法，该方法的线性检测范围为0.02至25 μM，检测限低至6.5 μg/L，同时具有出色的抗干扰能力和稳定性。该方法还应用于实际样品检测，水样中SM2的平均回收率范围为81.31%至97.11%，相对标准偏差（RSD）为0.57%至3.20%，与超高效液相色谱-串联质谱（UPLC-MS/MS）方法相比无显著差异，证明了良好的准确性和实用性。总而言之，MIL-101(Fe,Co)@MIP荧光传感检测方法操作简便、检测速度快，是一种适用于SM2快速检测的新方法。
