Doris P A
Clin Exp Hypertens A. 1985;7(10):1441-56. doi: 10.3109/10641968509073602.
Normotensive female Wistar Kyoto rats were studied to examine the effects of replacing drinking water with highly palatable saline solution at a concentration (0.5%) close to the maximum preferred concentration in these animals. A further group of animals was offered a calcium-supplemented diet in addition to substitution of drinking water with saline. Fluid consumption in animals drinking tap water was constant at between 35-40 ml/day throughout the six-month study period. In contrast, animals drinking 0.5% saline consumed 75-85 ml/day throughout the study period, irrespective of the calcium content of the diet. Voluntary consumption of these quantities of saline was associated with the development of a moderate hypertension, measured by tail cuff plethysmography, after two months of study. However, in animals consuming calcium-supplemented diets the hypertensive response disappeared after 3 months of study. Blood pressures were validated at the conclusion of the study by direct arterial cannulation and confirmed the presence of hypertension in saline drinking animals only when diets lacked calcium supplementation. No changes in blood ionized sodium concentration were associated with saline consumption; however, blood ionized calcium was significantly reduced in animals drinking saline, but not when calcium-supplemented diet was available. These studies suggest an interaction between sodium and calcium in the genesis of sodium-dependent hypertension.
对血压正常的雌性Wistar Kyoto大鼠进行了研究，以检验用浓度为0.5%（接近这些动物的最大偏好浓度）的高适口性盐溶液替代饮用水的效果。另一组动物除了用盐溶液替代饮用水外，还提供了补充钙的饮食。在整个为期六个月的研究期间，饮用自来水的动物的液体摄入量保持在每天35 - 40毫升之间。相比之下，饮用0.5%盐溶液的动物在整个研究期间每天摄入75 - 85毫升，与饮食中的钙含量无关。在研究两个月后，通过尾袖体积描记法测量，自愿摄入这些量的盐溶液与中度高血压的发展有关。然而，在摄入补充钙饮食的动物中，研究三个月后高血压反应消失。在研究结束时，通过直接动脉插管对血压进行了验证，结果证实只有在饮食缺乏钙补充时，饮用盐溶液的动物才会出现高血压。盐溶液摄入与血液中离子化钠浓度的变化无关；然而，饮用盐溶液的动物血液中离子化钙显著降低，但在有补充钙饮食时则不会。这些研究表明，在钠依赖性高血压的发生过程中，钠和钙之间存在相互作用。
