Gao Sheng-Chao, Zhang Zhen, Li Yong-Dong, Su Zhao-Ying, Dong Jin-Song, Zhang Ru-Meng, Zhou Wen, Ni Hong-Xia, Chen Yi, Liu Ying
Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, P.R. China.
Ningbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory of Virus Research, Ningbo 315010, P.R. China.
iScience. 2025 Jul 5;28(8):113069. doi: 10.1016/j.isci.2025.113069. eCollection 2025 Aug 15.
Exoelectrogenic biofilms is crucial for the energy generation and wastewater treatment by bioelectrochemical systems. In this study, two kinds of saline-alkali tolerant mixed exoelectrogenic cultures were enriched from biogas slurry. These mixed exoelectrogens produced current densities of 1,034 ± 30 and 974 ± 53 μA·cm, which were 1.68 and 1.58 times higher than PCA at pH 9.0, respectively. Under alkaline conditions with the addition of 1.5% NaCl, the maximum current densities for BS-N (546.66 ± 139.20 μA·cm) and BS-O (583 ± 22.91 μA·cm) were about 2.45 and 2.61 times higher than PCA, respectively. The BS-O exhibited a high power density of 1,109 ± 115 mW·m at pH 9.2 in a microbial fuel cell. The community analysis revealed Proteobacteria as the predominant phylum, with Firmicutes and Bacteroidetes as the dominant families within the biofilms. These findings provide valuable insights for further research on exoelectrogenesis in challenging environments.
产电生物膜对于生物电化学系统的能量产生和废水处理至关重要。在本研究中，从沼液中富集了两种耐盐碱混合产电培养物。这些混合产电菌产生的电流密度分别为1034±30和974±53μA·cm，在pH 9.0时分别比PCA高1.68倍和1.58倍。在添加1.5% NaCl的碱性条件下，BS-N（546.66±139.20μA·cm）和BS-O（583±22.91μA·cm）的最大电流密度分别比PCA高约2.45倍和2.61倍。在微生物燃料电池中，BS-O在pH 9.2时表现出1109±115 mW·m的高功率密度。群落分析表明，变形菌门是主要菌门，厚壁菌门和拟杆菌门是生物膜中的优势菌科。这些发现为在具有挑战性的环境中进一步研究产电作用提供了有价值的见解。
