蜂毒肽对自发性高血压大鼠培养皮肤成纤维细胞中钠和铷转运的影响。

The effect of melittin on Na+ and Rb+ transport in cultured skin fibroblasts of the spontaneously hypertensive rat.

作者信息

Kino M, Tamura H, Hopp L, Tokushige A, Searle B M, Aviv A

出版信息

Clin Exp Hypertens A. 1985;7(9):1283-99. doi: 10.3109/10641968509073591.

DOI:10.3109/10641968509073591

Abstract

Melittin effect on transport of Na+ and Rb+(K+ analog) was examined in cultured skin fibroblasts originating from the Spontaneously Hypertensive, Wistar Kyoto and Wistar rats. Melittin increased both Na+ (22Na+) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb+ uptake) in all three preparations. The effect of the toxin was maximal at a dose of 160-240ng/10(5) cells/ml. At this dose, fibroblasts of the Spontaneously Hypertensive rat demonstrated the greatest response to melittin with respect to the increase in Na+ and Rb+ fluxes and increase in the intracellular Na+ concentrations. It is concluded that melittin can be utilized as a probe to delineate subtle differences in the cellular regulation of Na+ and K+ in the Spontaneously Hypertensive rat as compared with its normotensive controls.

摘要

研究了蜂毒肽对源自自发性高血压大鼠、Wistar Kyoto大鼠和Wistar大鼠的培养皮肤成纤维细胞中Na⁺和Rb⁺（K⁺类似物）转运的影响。在所有这三种细胞制剂中，蜂毒肽均增加了Na⁺（²²Na⁺）摄取、⁸⁶Rb⁺外流以及Na⁺泵活性（哇巴因敏感的⁸⁶Rb⁺摄取）。毒素的作用在剂量为160 - 240ng/10⁵细胞/ml时最大。在此剂量下，就Na⁺和Rb⁺通量增加以及细胞内Na⁺浓度升高而言，自发性高血压大鼠的成纤维细胞对蜂毒肽表现出最大反应。得出的结论是，与正常血压对照相比，蜂毒肽可用作一种探针来描绘自发性高血压大鼠细胞中Na⁺和K⁺细胞调节方面的细微差异。