Meng Zixuan, Han Yuehua, Ruan Linda, Xu Chenming, Zhang Mengxiao, Liu Hao
School of Pharmacy, Bengbu Medical University, Bengbu, People's Republic of China.
Anhui Province Engineering Technology Research Center of Biochemical Pharmaceutical, Bengbu, Anhui Province, People's Republic of China.
Diabetes Metab Syndr Obes. 2025 Jul 29;18:2573-2584. doi: 10.2147/DMSO.S523821. eCollection 2025.
Establishing HepG2 cell insulin resistance models using glucosamine, high glucose with high insulin and palmitic acid and briefly evaluating them to provide reliable models for insulin resistance research.
Three methods were used to induce insulin resistance in HepG2 cells, and glucose uptake and consumption, glucose metabolism-related mRNA and p-AKT/AKT protein levels and RNA-seq were detected to compare the three induction methods.
Glucose consumption capacity was reduced after glucosamine and palmitic acid induction and did not change significantly after high glucose with high insulin induction. Glucose uptake capacity was not significantly changed after glucosamine and high glucose with high insulin induction and was reduced after palmitic acid induction. After high insulin stimulation, p-AKT/AKT levels were elevated in glucosamine and high glucose with high insulin induction and did not change significantly in palmitic acid induction. , , and were elevated after glucosamine and palmitic acid stimulation, and only PCK1 was elevated after high glucose with high insulin stimulation. The transcriptomes of cells induced by the three methods differed widely.
Treatment with 0.2 mM palmitic acid for 24 h is a simple and stable method to induce insulin resistance in HepG2 cells.
利用氨基葡萄糖、高糖高胰岛素以及棕榈酸建立HepG2细胞胰岛素抵抗模型，并对其进行简要评估，为胰岛素抵抗研究提供可靠模型。
采用三种方法诱导HepG2细胞产生胰岛素抵抗，检测葡萄糖摄取与消耗、葡萄糖代谢相关mRNA以及p-AKT/AKT蛋白水平，并进行RNA测序，以比较三种诱导方法。
氨基葡萄糖和棕榈酸诱导后葡萄糖消耗能力降低，高糖高胰岛素诱导后葡萄糖消耗能力无显著变化。氨基葡萄糖和高糖高胰岛素诱导后葡萄糖摄取能力无显著变化，棕榈酸诱导后葡萄糖摄取能力降低。高胰岛素刺激后，氨基葡萄糖和高糖高胰岛素诱导组p-AKT/AKT水平升高，棕榈酸诱导组无显著变化。氨基葡萄糖和棕榈酸刺激后， 、 和 升高，高糖高胰岛素刺激后仅磷酸烯醇式丙酮酸羧激酶1（PCK1）升高。三种方法诱导的细胞转录组差异很大。
用0.2 mM棕榈酸处理24小时是诱导HepG2细胞胰岛素抵抗的一种简单且稳定的方法。
