In this study, the mitochondrial genome data of were subjected to a multigene tandem method to elucidate its taxonomic status. The total length of the mitochondrial genome was determined to be 16,779 bp, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and the D-loop region. Overall, there was a strong AT bias and anti-G bias; different protein-coding genes exhibited different degrees of codon preference. The rest of the amino acids, with the exception of tRNA, of the 22 tRNAs were in one form, the secondary structure was incomplete, and the remaining tRNAs folded to form the secondary structure of a typical clover. Thirteen protein-coding genes of and were concatenated to explore their phylogenetic relationships, with as the outgroup. The results revealed that , , and were the most closely related. This research would supplement the mitochondrial genome data, serving as a molecular basis for safeguarding species, facilitating genetic classification, and investigating Acheilognathinae phylogeny.