BGI-Shenzhen, Shenzhen 518083, China.
China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China.
Nucleic Acids Res. 2019 Jun 20;47(11):e63. doi: 10.1093/nar/gkz173.
Mitochondrial genome (mitogenome) plays important roles in evolutionary and ecological studies. It becomes routine to utilize multiple genes on mitogenome or the entire mitogenomes to investigate phylogeny and biodiversity of focal groups with the onset of High Throughput Sequencing (HTS) technologies. We developed a mitogenome toolkit MitoZ, consisting of independent modules of de novo assembly, findMitoScaf (find Mitochondrial Scaffolds), annotation and visualization, that can generate mitogenome assembly together with annotation and visualization results from HTS raw reads. We evaluated its performance using a total of 50 samples of which mitogenomes are publicly available. The results showed that MitoZ can recover more full-length mitogenomes with higher accuracy compared to the other available mitogenome assemblers. Overall, MitoZ provides a one-click solution to construct the annotated mitogenome from HTS raw data and will facilitate large scale ecological and evolutionary studies. MitoZ is free open source software distributed under GPLv3 license and available at https://github.com/linzhi2013/MitoZ.
线粒体基因组（mitogenome）在进化和生态学研究中发挥着重要作用。随着高通量测序（HTS）技术的出现，利用线粒体基因组上的多个基因或整个线粒体基因组来研究焦点群体的系统发育和生物多样性已成为常规。我们开发了一个线粒体基因组工具包 MitoZ，它由从头组装、findMitoScaf（寻找线粒体支架）、注释和可视化的独立模块组成，可以从 HTS 原始读取中生成线粒体基因组组装以及注释和可视化结果。我们使用总共 50 个公开可用的线粒体基因组样本评估了它的性能。结果表明，与其他可用的线粒体基因组组装器相比，MitoZ 可以更准确地恢复更多全长的线粒体基因组。总的来说，MitoZ 提供了一种从 HTS 原始数据构建注释线粒体基因组的一键解决方案，将促进大规模的生态和进化研究。MitoZ 是免费的开源软件，根据 GPLv3 许可证发布，并可在 https://github.com/linzhi2013/MitoZ 上获得。
