Volpe J P, Hunter N, Basic I, Milas L
Clin Exp Metastasis. 1985 Oct-Dec;3(4):281-94. doi: 10.1007/BF01585082.
The spontaneous metastatic properties of six sarcomas and seven carcinomas syngeneic to C3Hf/Kam mice were investigated and the correlation between spontaneous metastasis, the lung colony forming efficiency (LCFE) of i.v. injected tumor cells, and s.c. tumor take was determined. The incidence and number of spontaneous metastases in the lung were determined in mice that had primary tumors in the leg removed 17 to 120 days earlier, depending on tumor type. There was a significant positive correlation between spontaneous metastasis and LCFE when all 13 tumors were compared, but the significance was lost when carcinomas and sarcomas were considered separately. No significant correlation between spontaneous metastasis and the s.c. tumor take was observed. Also, no correlation was found between LCFE and the s.c. tumor take of carcinomas, but there was a strong inverse relationship between these two properties of sarcomas. The number of cells shed from primary tumors was estimated and found to be more extensive in tumors with higher metastatic properties. Thus, in general, highly metastatic tumors were characterized by a high LCFE and a significant cell shedding. Furthermore, LCFE was greatly increased by treatment of animals with cyclophosphamide and by admixing heavily irradiated tumor cells to viable cells, implying that local environmental factors are important in determining the establishment of tumor cell clonogens into metastasis.
研究了与C3Hf/Kam小鼠同基因的6种肉瘤和7种癌的自发转移特性，并确定了自发转移、静脉注射肿瘤细胞的肺集落形成效率（LCFE）和皮下肿瘤接种之间的相关性。根据肿瘤类型，在17至120天前切除腿部原发性肿瘤的小鼠中，确定肺中自发转移的发生率和数量。当比较所有13种肿瘤时，自发转移与LCFE之间存在显著正相关，但当分别考虑癌和肉瘤时，这种相关性消失。未观察到自发转移与皮下肿瘤接种之间存在显著相关性。此外，未发现癌的LCFE与皮下肿瘤接种之间存在相关性，但肉瘤的这两种特性之间存在强烈的负相关。估计了原发性肿瘤脱落的细胞数量，发现具有较高转移特性的肿瘤中细胞脱落更为广泛。因此，一般来说，高转移性肿瘤的特征是高LCFE和显著的细胞脱落。此外，用环磷酰胺治疗动物以及将大量照射过的肿瘤细胞与活细胞混合会大大提高LCFE，这意味着局部环境因素在决定肿瘤细胞克隆原形成转移方面很重要。
