Jiang Wang, Liang Yi, Han Min, He Wenhua, Chen Kun, Deng Chongtian, Shen Yueming
Department of Digestive Diseases, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, 161 Shaoshan Road, Changsha, 410000, China.
Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, 35 Jiefang Road, Hengyang, 421000, China.
Open Life Sci. 2025 Aug 1;20(1):20251130. doi: 10.1515/biol-2025-1130. eCollection 2025.
RNF144 family proteins, including RNF144A and RNF144B, members of the RING-between-RING domain-containing ubiquitin E3 ligase family, serve as critical regulators of protein ubiquitination. Despite increasing research attention in recent years, particularly regarding their distinct functional roles in pathophysiological processes, a comprehensive synthesis of existing findings remains absent. To address this knowledge gap, we conducted a systematic literature search in PubMed using the following query terms: "RNF144," "RNF144A," "RNF144B," "PIR2," "IBRDC2," and "P53RFP." This review systematically examines current evidence regarding the molecular mechanisms and pathophysiological significance of RNF144A/B across various disease systems. Through critical analysis of structural characteristics, substrate interactions, and signaling pathways, we aim to clarify their dual roles in cellular homeostasis and disease pathogenesis. This synthesis not only consolidates current understanding but also identifies key knowledge gaps requiring further investigation, particularly regarding isoform-specific functions and therapeutic targeting potential.
RNF144家族蛋白，包括RNF144A和RNF144B，属于含RING结构域的泛素E3连接酶家族成员，是蛋白质泛素化的关键调节因子。尽管近年来研究关注度不断提高，尤其是关于它们在病理生理过程中的独特功能作用，但目前仍缺乏对现有研究结果的全面综合。为了填补这一知识空白，我们在PubMed中使用以下检索词进行了系统的文献检索：“RNF144”、“RNF144A”、“RNF144B”、“PIR2”、“IBRDC2”和“P53RFP”。本综述系统地研究了目前关于RNF144A/B在各种疾病系统中的分子机制和病理生理意义的证据。通过对结构特征、底物相互作用和信号通路的批判性分析，我们旨在阐明它们在细胞稳态和疾病发病机制中的双重作用。这一综合不仅巩固了当前的认识，并确定了需要进一步研究的关键知识空白，特别是关于异构体特异性功能和治疗靶向潜力方面。
