Kim Woo-Ju, Kim Yoonbin, Koysuren Begum, Nitin Nitin
Department of Food Science and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea.
Research Institute of Food and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea.
Curr Res Food Sci. 2025 Jul 22;11:101155. doi: 10.1016/j.crfs.2025.101155. eCollection 2025.
Plant protein-based, 3D-structured scaffolds that mimic the mechanical properties of conventional meat products while supporting cytocompatibility and cellular growth are essential for developing alternative meat products. This study evaluates the development of pea protein isolate (PPI)-derived 3D foam scaffolds for cellular agriculture using heat and tannic acid (TA) as cross-linking agents. Infrared (IR) spectroscopy and scanning electron microscopy (SEM) analyses demonstrated the effects of thermal and TA treatments on the cross-linking of the proteins and microstructure of the PPI foam scaffold. Textural profile analysis (TPA) revealed that the mechanical properties of the fabricated scaffolds are comparable to those of conventional meats and meat analogues, and the addition of TA increased the hardness and reduced the cohesion of the scaffold. The result of the digestibility test demonstrated that the scaffolds exhibited a free amino acid release profile similar to that of pea protein gels. The cytocompatibility of the 3D foam scaffolds was evaluated using C2C12 myoblast cells as a model cell line. Rapid adhesion and significant proliferation of the cells were observed on the 3D foam scaffolds. Overall, the findings of this study highlight the potential of plant-based, 3D foam scaffolds for cellular agriculture applications.
基于植物蛋白的3D结构支架，在支持细胞相容性和细胞生长的同时，模拟传统肉类产品的机械性能，对于开发替代肉类产品至关重要。本研究评估了以豌豆分离蛋白（PPI）为原料，使用热和单宁酸（TA）作为交联剂，用于细胞农业的3D泡沫支架的开发。红外（IR）光谱和扫描电子显微镜（SEM）分析证明了热和TA处理对蛋白质交联以及PPI泡沫支架微观结构的影响。质地剖面分析（TPA）显示，所制备支架的机械性能与传统肉类和肉类替代品相当，添加TA增加了支架的硬度并降低了内聚性。消化率测试结果表明，支架呈现出与豌豆蛋白凝胶相似的游离氨基酸释放曲线。以C2C12成肌细胞作为模型细胞系评估了3D泡沫支架的细胞相容性。在3D泡沫支架上观察到细胞快速黏附并显著增殖。总体而言，本研究结果突出了基于植物的3D泡沫支架在细胞农业应用中的潜力。
