Alfaifi Mohammed, Mansour Adel Abo, Yadav Bijesh, Ali Khan Imran
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Department of Population Health, Division of Biostatistics, King Abdullah International Medical Research Center, Ministry of National Guard-Health Affairs, Riyadh, Saudi Arabia.
Int J Womens Health. 2025 Jul 28;17:2335-2352. doi: 10.2147/IJWH.S510344. eCollection 2025.
Gestational Diabetes Mellitus (GDM) is defined as impaired glucose intolerance resulting in hyperglycemia. SIRT1 deficiency and single nucleotide polymorphisms (SNPs) were also related to diabetes. No documented studies were carried out between GDM and SNPs and it is also not confirmed whether SNPs had a role in GDM women.
This study was designed to explore the molecular association carried out between GDM women and gene SNPs present in the Saudi Arabia. This is a case-control study carried out in Capital city of Saudi Arabia.
METHODS/DESIGN: Serum samples were used for studying biochemical parameters, genotyping and Sanger sequencings for rs4746720 and rs10823112 SNPs. Statistical analysis was carried out between GDM and non-GDM women for anthropometric, biochemical and molecular data.
A total of 120 GDM and 120 non-GDM women were recruited based on inclusion and exclusion criteria. Different forms of glucose values have confirmed the statistical association levels between GDM and non-GDM women (p < 0.05). Penalized logistic regression analysis showed the positive association with FBG, OGTT-2hr and HDLc levels (p < 0.05) in GDM women. The rs4746720 SNP was associated with GDM (p = 0.01). ANOVA analysis has confirmed the strong association with FBG (p = 0.002), PPBG (p = 0.0001) and HDLc (p = 0.0003) levels in rs4746720 SNP; while in rs10823112 SNP, both PPBG (p = 0.0001) and HDLc (p = 0.01) were associated. Linkage disequilibrium and GMDR analysis showed significant associations (p < 0.05).
This study confirms rs4746720 SNP and glucose levels played a role in this study.
妊娠期糖尿病（GDM）被定义为导致高血糖的葡萄糖耐量受损。沉默调节蛋白1（SIRT1）缺乏和单核苷酸多态性（SNP）也与糖尿病有关。目前尚未有关于GDM与SNP之间的文献研究，SNP在GDM女性中是否起作用也未得到证实。
本研究旨在探讨沙特阿拉伯GDM女性与基因SNP之间的分子关联。这是一项在沙特阿拉伯首都进行的病例对照研究。
方法/设计：血清样本用于研究生化参数、基因分型以及对rs4746720和rs10823112 SNP进行桑格测序。对GDM和非GDM女性的人体测量、生化和分子数据进行统计分析。
根据纳入和排除标准，共招募了120名GDM女性和120名非GDM女性。不同形式的血糖值证实了GDM和非GDM女性之间的统计关联水平（p < 0.05）。惩罚逻辑回归分析显示，GDM女性的空腹血糖（FBG）、口服葡萄糖耐量试验2小时血糖（OGTT-2hr）和高密度脂蛋白胆固醇（HDLc）水平呈正相关（p < 0.05）。rs4746720 SNP与GDM相关（p = 0.01）。方差分析（ANOVA）证实，rs4746720 SNP与FBG（p = 0.002）、餐后血糖（PPBG，p = 0.0001）和HDLc（p = 0.0003）水平有很强的相关性；而在rs10823112 SNP中，PPBG（p = 0.0001）和HDLc（p = 0.01）均相关。连锁不平衡和基因多因素降维分析（GMDR）显示出显著相关性（p < 0.05）。
本研究证实rs4746720 SNP和血糖水平在本研究中发挥了作用。
