Fan Li, Wei Yingying, Chen Yi, Jiang Shu, Xu Feng, Elsherbiny Elsherbiny A, Shao Xingfeng
State Key Laboratory for the Quality and Safety of Agro-products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Engineering, Ningbo University, Ningbo 315800, China.
College of Biological Environment, JiYang College of Zhejiang A&F University, Zhuji 311800, China.
J Agric Food Chem. 2025 Aug 13;73(32):20375-20384. doi: 10.1021/acs.jafc.5c06129. Epub 2025 Aug 4.
Epinecidin-1 (EPI), an antifungal peptide, has specific anti- activity , but , it requires a very high concentration to efficiently manage postharvest gray mold. Our work aimed to improve the antifungal ability of EPI . A set of EPI analogues, generated by arginine substitution on the polar surface of EPI, was acquired. The biological properties of EPI and its derivatives were evaluated. Among the tested EPI analogues, the modified peptide EPI-4 demonstrated the most favorable characteristics, with higher anti- activity (the MIC value was 6 μmol/L compared to 12.5 μmol/L for EPI) and the lowest cytotoxicity. , EPI-4 disrupted the ultrastructure and cell membrane integrity of , induced more ROS accumulation, and enhanced the DNA binding ability of by twofold. More importantly, experiments confirmed that EPI-4 could effectively control postharvest gray mold of peach fruit at 50 μmol/L, and its control effect was significantly better than that of the template peptide EPI. Our findings suggest that EPI-4 demonstrates considerable promise as a valid compound for regulating gray mold disease in postharvest peaches.
表皮菌素-1（EPI）是一种抗真菌肽，具有特定的抗菌活性，但其需要非常高的浓度才能有效防治采后灰霉病。我们的工作旨在提高EPI的抗真菌能力。通过在EPI的极性表面进行精氨酸取代，获得了一组EPI类似物。对EPI及其衍生物的生物学特性进行了评估。在测试的EPI类似物中，修饰后的肽EPI-4表现出最有利的特性，具有更高的抗菌活性（MIC值为6 μmol/L，而EPI为12.5 μmol/L）和最低的细胞毒性。EPI-4破坏了（此处原文似乎缺失相关对象）的超微结构和细胞膜完整性，诱导了更多的活性氧积累，并使（此处原文似乎缺失相关对象）的DNA结合能力提高了两倍。更重要的是，实验证实EPI-4在50 μmol/L时能有效防治桃果实采后灰霉病，其防治效果明显优于模板肽EPI。我们的研究结果表明，EPI-4作为一种调控采后桃果实灰霉病的有效化合物具有很大的前景。
